INFORMATION REGARDING CARBON DISULFIDE AND BROMINE

http://toxnet.nlm.nih.gov/cgi-bin/sis/search/f?./temp/~Q0VHU4:1
Source: Occupational Diseases A Guide to Their Recognition, W. M. Gafafer, Editor, U.S. Department of Health, Education and Welfare, Washington, D. C., pages 107-108, 3 references, 19641964

Abstract:

 
The harmful effects of carbon-disulfide (75150), and the types of workers likely affected by it are listed. The local effects included eye irritation and also involve the nose, and skin. Routes of entry include inhalation of vapor and percutaneous absorption of liquid or vapor. Systemic effects act mainly on the nervous system, leading to neuritis, transient mental aberrations, mania, depression, hallucinations, and other abnormalities. Gastric disturbances are common, and chronic intoxication may lead to heart, liver, and kidney disorders. Special diagnostic tests include analysis of urine and blood. Recommended threshold limit value is 20ppm, or 60 milligrams per cubic meter of air. Workers engaged in the making of acetylene (74862), ammonium salt, bromine, carbanilide, carbon-tetrachloride (56235), cellophane, cement, rubber, dry cleaners, enamel, explosives, processed fat, fumigants, flotation agents, glass, glue, processed iodine, lacquer, matches, processed oil, paint, paint removers, paraffin, pesticide, processed phosphorus, putty, preservatives, rayon, resin, rocket fuel, rubber cement, rubber dryers, processed selenium, smokeless powder, textiles, vacuum tubes, tallow, processed sulfur, varnish, varnish removers, vulcanizing materials and processed wax are likely to be affected by carbon-disulfide.

http://toxnet.nlm.nih.gov/cgi-bin/sis/search/f?./temp/~Jf5B89:2
CARBON DISULFIDE

CASRN: 75-15-0

Probable Routes of Human Exposure :

Occupations with potential exposure to carbon disulfide: Acetylene workers, ammonium salt makers, bromine processors, carbonilide makers, carbon disulfide workers, carbon tetrachloride makers, cellophane makers, rubbershoe cementers, coal tar distillers, degreasers, drycleaners, dyestuff makers, electroplaters, enamelers, enamel makers, explosive workers, fat processors, floatation-agent makers, fumigant workers, glassmakers, glue workers, iodine processors, chemical laboratory workers, lacquer makers, matchmakers, oil processors, optical glassmakers, painters, paintmakers, paint-remover makers, paraffin workers, pesticide makers, phospherus processors, preservative makers, putty makers, rayon makers, resin makers, rocket-fuel makers, rubber- cement makers, rubber dryers, rubber makers, rubber reclaimers, sclenium processor, tallowmakers, textile makers, vacuum-tube makers, varnish-remover makers, veterinarians, vulcanizers, and wax processors.
[NIOSH; Criteria Document: Carbon Disulfide p.185 (1977) DHEW Pub. NIOSH 77-156]**PEER REVIEWED**

Artificial Pollution Sources :

Carbon disulfide's production and use in the manufacture of rayon, carbon tetrachloride, xanthogenates, soil disinfectants, electronic vacuum tubes, and as a solvent for phosphorus, sulfur, selenium, bromine, iodine, fats, resins, and rubbers(1) may result in its release to the environment through various waste streams(SRC).
[(1) Budavari S; The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. 12th ed. Whitehouse Station, NJ: Merck and Co Inc p 295 (1996)]**PEER REVIEWED**

Synonyms :

KOHLENDISULFID (SCHWEFELKOHLENSTOFF) (GERMAN)
**PEER REVIEWED**

Synonyms :

SCHWEFELKOHLENSTOFF (GERMAN)
**PEER REVIEWED**

Major Uses :

MFR OF RAYON, CARBON TETRACHLORIDE, XANTHOGENATES, SOIL DISINFECTANTS, ELECTRONIC VACUUM TUBES; SOLVENT FOR PHOSPHORUS, SULFUR, SELENIUM, BROMINE, IODINE, FATS, RESINS, & RUBBERS.
[Budavari, S. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 1996.296]**PEER REVIEWED**

Environmental Fate/Exposure Summary :

Carbon disulfide's production and use as a solvent and a chemical intermediate may result in its release to the environment through various waste streams. If released to air, a vapor pressure of 359 mm Hg at 25 deg C indicates carbon disulfide will exist solely as a vapor in the ambient atmosphere. Vapor-phase carbon disulfide will be degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals; the half-life for this reaction in air is estimated to be 5.5 days. Carbon disulfide has a weak UV adsorption band at 317 nm, suggesting a potential for direct photolysis. If released to soil, carbon disulfide is expected to have moderate mobility based upon an estimated Koc of 270. Volatilization from moist soil surfaces is expected to occur based upon a Henry's Law constant of 1.44X10-2 atm-cu m/mole at 24 deg C. Carbon disulfide may potentially volatilize from dry soil surfaces given its vapor pressure. If released into water, some adsorption of carbon disulfide to suspended solids and sediment in the water column is expected based upon the estimated Koc. Volatilization from water surfaces is expected to be an important fate process based upon carbon disulfide's Henry's Law constant. Estimated volatilization half-lives for a model river and model lake are 2.6 hours and 3.5 days, respectively. BCFs of <6.1 and <60 in carp suggest bioconcentration in aquatic organisms is low to moderate. Occupational exposure to carbon disulfide may occur through inhalation and dermal contact with this compound at workplaces where carbon disulfide is produced or used. As carbon disulfide occurs ubiquitously in the environment, the general population is exposed to this compound. Primary routes of exposure to carbon disulfide are through inhalation of ambient air or ingestion of fruits, vegetables, and other food products containing this compound. (SRC)
**PEER REVIEWED**

Food Survey Values :

Seven samples of lima beans, five of common beans, two of lentils and one sample of mung beans, soybeans, and split peas that were analyzed contained a mean carbon disulfide concn of 2.3 ppb; range was 1.8 to 3.1 ppb(1). Some of these samples were obtained from health stores and one was from a home garden where it was grown without the use of herbicides or insecticides(1). Stored grain has been fumigated with carbon disulfide in the past. The carbon disulfide concn in 9 samples of wheat, range from 64 to 7500 ppb although none was found in samples of corn, oats, corn meal, and corn grits(2). While one sample of bleached flour contained 23 ppb, other samples tested, including corn muffin mix, cake mixes, dried lima beans, noodles and rice, were free of the fumigant(2). With the exception of granola, which contained 11 ppb of carbon disulfide, none of the 18 samples of table-ready food items tested contained carbon disulfide(3). The items tested contained representative samples of most classes of food(3). In addition, samples of butter and margarine, cheese, peanut butter, and highly processed foods from the US FDA's market basket survey contained no carbon disulfide(3). However some ready-to-eat food products, specifically two samples of corn chips, and oat ring cereal contained 80, 230, and 95 ppb of carbon disulfide, respectively(3). In an FDA Survey of 549 food items for fumigant residues, 7 were found to contain carbon disulfide(4). The avg concn of carbon disulfide residues was 738 ppb(4). Carbon disulfide has been identified as a volatile component in chicken and beef flavor(5).
[(1) Lovegren NV et al; J Agric Food Chem 27: 851-3 (1979) (2) Heikes DL, Hopper ML; J Assoc Off Anal Chem 69: 990-8 (1986) (3) Heikes DL; J Assoc Off Anal Chem 70: 215-77 (1987) (4) Daft JL; Sci Tot Environ 100: 501-18 (1991) (5) Shahidi F et al; CRC Crit Rev Food Sci Nature 24: 141-2431 (1986)]**PEER REVIEWED**

Natural Pollution Sources :

The ocean appears to be a major global source of carbon disulfide(1-3). Current data suggest that coastal areas and other areas of high biological productivity have greater fluxes of carbon disulfide than the open ocean(2). Emissions from the oceans have been estimated to be 6X10+11 g/yr(2). The microbial reduction of sulfates in soil produces fluxes of carbon disulfide. The annual global emission from this source has been estimated to be 9X10+11 g(2). Other natural sources include volcanic emissions, estimated to be 2X10+10 g/yr, and marshlands, estimated emissions 1X10+11 g/yr(2). Fluxes of carbon disulfide from a salt marsh was measured as 0.2 and 1.13 g Sulfur/sq m-yr and inland soil 0.001 g Sulfur/sq m-yr(5). However, the overall contribution of inland soil is much higher(5). Some higher plants are known to emit carbon disulfide(7,9). The principle source is the tree roots(9). Emissions from tidal marshes is greatest when the soil moisture is at field capacity(4). Diurnal variation in emissions rates correlate loosely with soil temperature and solar irradiation(6). Carbon disulfide emissions rates were highest in the early- to mid-afternoon and lowest during the early morning. Carbon disulfide emissions from a temperate pine forest increased nine-fold when nitrogen fertilizers were added(8). However, similar increases were not observed in a temperate hardwood forest. Carbon disulfide was only emitted briefly from a normally aerobic loam soil and only in the saturated static forest silty loam soil did not emit any carbon disulfide(4).
[(1) Carroll MA; J Geophys Res 90: 10483-6 (1985) (2) Khalil MAK; Rasmussen RA; Atmos Environ 18: 1805-31 (1984) (3) Lovelock JE; Nature 248: 625-6 (1974) (4) Farwell SO et al; Soil Biol Biochem 11: 411-5 (1979) (5) Aneja VP et al; J Air Pollut Control Assoc 32: 803-7 (1982) (6) Cooper WJ et al; Atmos Environ 21: 491-5 (1987) (7) Aneja VP; J Air Waste Manage Assoc 40: 469-76 (1990) (8) Melillo JM, Steudler PA; J Atmos Chem 9: 411-7 (1989) (9) Kelly DR, Smith NA; Adv Microb Ecol 11: 345-85 (1990)]**PEER REVIEWED**

Preventive Measures :

... Clothing wet with liquid carbon disulfide should be placed in closed containers for storage until it can be discarded or until provision is made for the removal of carbon disulfide from the clothing. If the clothing is to be laundered or otherwise cleaned to remove the carbon disulfide, the person performing the operation should be informed of carbon disulfide's hazardous properties. Any clothing which becomes wet with liquid carbon disulfide should be removed immediately and not reworn until the carbon disulfide is removed from the clothing. Skin that becomes contaminated with carbon disulfide should be promptly washed or showered with soap or mild detergent and water to remove any carbon disulfide.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.3]**PEER REVIEWED**

Medical Surveillance :

Whole Blood: Reference Ranges: Normal - None detected; Exposed - Not established; Toxic - Not established. The assessment of carbon disulfide exposure can be accomplished through measurement of carbon disulfide. However, data obtained from measurement of carbon disulfide in blood have not shown a consistent correlation with exposure. One reason that carbon disulfide in blood is a poor indicator of exposure is due to its rapid clearance from this tissue. A compounding factor in blood measurement is that there are two species of carbon disulfide in the blood: "Free" which is unbound carbon disulfide that is dissolved in the plasma; and "bound" or "acid-labile" carbon disulfide, which is dissolved in plasma lipids or bound proteins. The equilibrium of these forms differs from individual to individual, rendering blood tests less reliable. However, a new headspace gas chromatography method that is being developed for acid labile carbon disulfide may prove to be more reliable.
[Ryan, R.P., C.E. Terry (eds.). Toxicology Desk Reference 4th ed. Volumes 1-3. Taylor & Francis, washington, D.C. 1997.623]**PEER REVIEWED**

National Toxicology Program Studies :

Carbon disulfide (CS), a widely used industrial chemical, was evaluated for toxic and teratogenic effects in artificially inseminated New Zealand White (NZW) rabbits. Carbon disulfide (0, 25, 75 and 150 mg/kg/day, po) in corn oil was administered in a volume of 1 ml/kg body weight on gestational days (gd) 6 through 19. Females were weighed and observed during daily treatment and at 4 hours post-dosing for clinical signs of toxicity. At sacrifice on gd 30, a total of 23-28 confirmed-pregnant females per treatment group were evaluated. The gravid uterus for each dam was weighed and the number of implantation sites, and live, dead, or resorbed fetuses were recorded. All live fetuses were weighed and examined for external, visceral and skeletal malformations. The mortality rate for treated females was 3.5% (1/29), 0% (0/26), 3.3% (1/30), and 7.1% (2/28) for the vehicle control through high dose, respectively. Maternal body weight on gd 0, gd 6 (i .e., prior to the initiation of treatment), gd 12 (i.e., midway through the treatment period) and gd 30 (i.e., immediately prior to sacrifice) did not differ significantly among treatment groups. On gd 19 (i.e., the final day of treatment) maternal body weight was decreased across treatment groups in a dose-related manner with 150 mg/kg/day CS dams exhibiting body weights significantly below vehicle controls. Maternal weight gain during treatment, maternal weight gain during gestation and gravid uterine weight were each decreased in a dose-related manner. Maternal weight gain during the treatment period was significantly below controls for dams treated with 75 or 150 mg/kg/day carbon disulfide. Gestational weight gain was below controls only for the high-dose group. Absolute weight gain (i.e. maternal gestational weight gain minus gravid uterine weight) did not differ significantly among treatment groups. Both absolute and relative maternal liver weight were increased in a dose-related manner with statistically significant increases above vehicle control observed in the 75 and 150 mg/kg/day carbon disulfide groups. Since maternal body weight at sacrifice did not differ among treatment groups, the increase in absolute and relative maternal liver weight appears to reflect a treatment related hepatic response, but the data collected did not allow further characterization of this response. The percentage per litter of resorbed, nonlive (i.e., dead plus resorbed) or affected (i.e., nonlive plus malformed) fetuses was increased in a dose-related manner, and all carbon disulfide-treated groups were significantly above vehicle controls on these measures. The proportion of litters with one or more resorbed, nonlive or affected fetuses also increased in a dose-related manner, but only the high-dose group (150 mg/kg/day, carbon disulfide) was significantly above vehicle controls on these measures. No statistically significant differences among treatment groups were observed in the percentage of dead fetuses (i.e., fetuses weighing approx 10 g with discernible digits, but showing no vital signs at uterine dissection) per litter or in the proportion of litters with one or more dead fetuses. Among those litters containing live fetuses, there were no differences among dose groups in the proportion of males per live litter. The number of live fetuses per litter, as well as average fetal body weight per live litter, were reduced in a dose-related manner, with carbon disulfide 150 litters significantly smaller than controls on both measures. The percentage of fetuses malformed per litter, but not the proportion of litters with one or more malformed fetuses, differed significantly among treatment groups and a clear dose-effect relationship was observed. In conclusion, carbon disulfide (25, 75 or 150 mg/kg/day, po) administered on gd 6 through 19, produced dose-related maternal and fetal toxicity, and increased the incidence of malformed fetuses in New Zealand White rabbits relative to the vehicle control group. The incidence of resorptions was significantly increased at all doses tested (i.e., 12.30%, 32.47%, 41.60% and 61.16% resorbed in the vehicle through high-dose, respectively), but the incidence of malformations in the control group (5.72%) was significantly exceeded only in the high dose group (19.51% malformed fetuses per litter).
[Department of Health & Human Services/National Institute of Environmental Health Sciences, National Toxicology Program; Teratologic Evaluation of Carbon Disulfide (CAS No. 75-15-0) Administered to New Zealand White Rabbits on Gestational Days 6 Through 19, NTP Study No. TER84054 (February 24, 1984) available at http://ntp-server.niehs.nih.gov/htdocs/pub-TT0.html as of August 15, 2002]**QC REVIEWED**

Absorption, Distribution & Excretion :

Six human volunteers were exposed to 10 and 20 ppm carbon disulfide at rest and to 3 and 10 ppm carbon disulfide under a 50 W level of physical exercise during four consecutive periods of 50 min. Every 5 min a sample was taken from the mixed exhaled air in which the concentration of carbon disulfide was determined. It was established that only an apparent steady state was reached during this exposure period. The retention values were established as 0.374 (SD= 0.106; n= 239) for exposure to 10 ppm carbon disulfide at rest and as 0.410 (SD= 0.103; n= 239) for exposure to 20 ppm carbon disulfide at rest. During exposure to 10 ppm and 3 ppm carbon disulfide, combined with a 50 W level of physical exercise, the retention values decreased to 0.286 (SD= 0.083; n= 239) and 0.277 (SD= 0.049; n= 239) respectively. The respiratory uptake of carbon disulfide (mg carbon disulfide) proved significantly influenced by the amount of body fat estimated from skinfold thickness measurements.
[Rosier J et al; Int Arch Occup Environ Health 59 (3): 233-42 (1987)]**PEER REVIEWED**

Metabolism/Metabolites :

Carbon disulfide is metabolized by two distinctly different pathways: ability of carbon disulfide to spontaneously react with free amine and sulfhydryl groups of amino acids and polypeptides, and microsomal metabolism of carbon disulfide to reactive intermediates capable of covalently binding to tissue macromolecules. Dithiocarbamates are formed during in vitro incubations of carbon disulfide with blood; treatment of the dithiocarbamate products with acid and heat liberates carbon disulfide. Tissue concentrations of acid labile metabolites exceed those of free carbon disulfide at the end of an 8 hour, 2 mg/l carbon disulfide inhalation exposure. Acid labile metabolites may also accumulate in the body after repeated carbon disulfide exposure.
[Bus JS; Neurotoxicology 6 (4): 73-80 (1985)]**PEER REVIEWED**

Metabolism/Metabolites :

In an attempt to further define the mechanisms involved in carbon disulfide metabolism, the relationship between carbon disulfide and carbonyl sulfide metabolism was explored in male Sprague-Dawley rat hepatocytes and liver microsomes. Pretreatment of animals with cobaltous chloride or phenobarbital decreased or increased, respectively, the extent of carbon disulfide metabolism by hepatic microsomes, as determined by the formation of carbonyl sulfide and nonvolatile sulfur compounds. Carbon disulfide metabolism in microsomes was biphasic in that there was an initial period of rapid metabolite formation followed by a period of slower metabolism. Carbon disulfide metabolism in hepatocytes was biphasic as well. SKF-525A significantly inhibited carbon disulfide metabolism in hepatocytes and microsomes from phenobarbital treated rats. Acetazolamide did not significantly inhibit carbon disulfide metabolism regardless of the metabolite studied.
[Chengelis CP, Neal RA; Biochemical Pharmacology 36 (3): 363-8 (1987)]**PEER REVIEWED**

Atmospheric Concentrations :

SOURCE DOMINATED: Carbon disulfide was detected in air samples from municipal solid waste composting facilities at a maximum concn of 150 ug/cu m; average concns of 5, 9, 8, and 6 ug/cu m were detected in air samples collected near newly formed compost, from one-fifth to four-fifths through the active composting region, at the end of the composting region, and outside the active composting region, respectively(1). Carbon disulfide concns in the air of the spinning department of a viscose rayon fiber factory ranged from 0.1 to 8.5 ppm, mean 3 ppm; in the pause cabin, carbon disulfide levels ranged from 0.01 to 1 ppm, mean 0.04 ppm(3). Personal air samples collected from the breathing zone, shoulder area, ranged from 2 to 39 ppm (TWA); corresponding samples drawn from the mask ranged from 1 to 14 ppm(2). Carbon disulfide levels in ambient air samples from a factory producing viscose sheeting ranged from 0.2 to 9 ppm, mean 4 ppm; concns in personal air samples ranged from 3 to 7 ppm (TWA)(2). Air samples collected from personal sampling tubes of workers during the production of viscose rayon fibers ranged from 1.0 to 148 mg/cu m(3). Mean carbon disulfide concns for workers in the viscose industry measured by passive personal air monitoring were (department): 3.42 (spool spinning), 6.57 (spinning of technical rayon), 12.07 (washing), 3.63 (post-treatment), and 1.94 (second aging) ppm(4). Mean carbon disulfide concns for workers in the viscose industry measured by active personal air monitoring were (department): 4.18 (spool spinning), 6.05 (spinning of technical rayon), 9.54 (washing), and 2.00 (second aging) ppm(4). Mean carbon disulfide concns for workers in the viscose industry measured by stationary air monitoring were (department): 0.91 (spool spinning), 7.51 (spinning of technical rayon), 28.07 (washing), 1.63 (post-treatment), and 2.30 (second aging) ppm(4).
[(1) Eitzer BD; Environ Sci Technol 29: 896-902 (1995) (2) Riihimaki V et al; Am J Ind Med 2: 85-97 (1992) (3) Kitamura S et al; Int Arch Occup Environ Health 65: S177-80 (1993) (4) Drexler H et al; Int Arch Occup Environ Health 65: 359-65 (1994)]**PEER REVIEWED**

Environmental Abiotic Degradation :

The rate constant for the vapor-phase reaction of carbon disulfide with photochemically-produced hydroxyl radicals is 2.9X10-12 cu cm/molecule-sec at 24 deg C(1). This corresponds to an atmospheric half-life of about 5.5 days at an atmospheric concn of 5X10+5 hydroxyl radicals per cu cm(1,SRC). Observed temporal variability and vertical gradients suggest that the tropospheric lifetime of carbon disulfide is quite short(2). If the atmospheric concn of carbon disulfide at 6.1 km altitude is typical for marine boundary layer levels, the sharp decrease in concn at higher altitudes supports the concept of a photochemical lifetime of a month or less in the troposphere(2). The tropospheric half-life determined from estimates of sources and global burdens of the chemical is 8.9 days(4). Carbon disulfide has a weak UV adsorption band at 317 nm(3); however, photolysis is not considered to be a significant loss mechanism for the chemical(4). The rate constant for the vapor-phase reaction of carbon disulfide with atomic oxygen is 3.6X10-12 cu cm/molecule- sec at 25 deg C(5). This corresponds to an atmospheric half-life of about 8.9 days(SRC) assuming an atmospheric concn of atomic oxygen is 2.5X10+5 radicals/cu cm(5,6). The rate constant for the reaction of carbon disulfide with hydroxyl radicals in aqueous solution is 8.0X10+9 L/mol sec at pH 7.6(7). This corresponds to a half-life of about 100 days(SRC) at an avg aqueous hydroxyl radical concn of 1X10-17 mol/l(8).
[(1) Arnts RR et al; J Air Pollut Control Assoc 39: 453-60 (1989) (2) Carroll MA; J Geophys Res 90: 10483-6 (1985) (3) Sadtler Index; Philadelphia, PA: Sadtler Research Lab (NA) (4) Khalil MAK, Rasmussen RA; Atmos Environ 18: 1805-31 (1984) (5) Hampson RF; Chemical, kinetic and photochemical data sheets for atmospheric reactions FAA-EE-80-17 (1980) (6) Graedel TE; Chemical Compounds in the Atmosphere. NY,NY: Academic Press (1978) (7) Buxton GV et al; J Phys Chem Ref Data 17: 513-882 (1988) (8) Mill T et al; Science 207: 886-7 (1980)]**PEER REVIEWED**

Medical Surveillance :

A complete history and physical examination: The purpose is to detect existing conditions that might place the exposed employee at increased risk, and to establish a baseline for future health monitoring. Examination of the central and peripheral nervous systems, eyes, cardiovascular system, kidneys, and liver should be stressed. The skin should be examined for evidence of chronic disorder. Since kidney damage has been observed in humans exposed to carbon disulfide, a urinalysis should be obtained to determine, at a minimum, specific gravity, albumin and glucose content, along with a microscopic /examination of/ centrifuged sediment. Since liver damage has been observed in humans exposed to carbon disulfide, a profile of liver function should be obtained by using a medically acceptable array of biochemical tests. An electrocardiogram: carbon disulfide has caused arrhythmias and electrocardiographic changes in humans. Periodic surveillance is indicated. Carbon disulfide has caused ocular changes in humans. An ophthalmic examination should be performed, including visual acuity. Workers should be informed of potential undesirable effects of exposure to carbon disulfide on reproduction (such as spermatic deficiences, menstrual disorders, and spontaneous abortions).
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.2]**PEER REVIEWED**

Absorption, Distribution & Excretion :

/A study was conducted to determine the/ biochemical changes due to carbon disulfide in 11 male New Zealand white rabbits. The rabbits were exposed to carbon disulfide by inhalation for 6 hours/day, 5 days/week, for up to 38 weeks. Concentrations of carbon disulfide were 250 ppm (775 mg/cu m) during the first 16 weeks, 500 ppm (1,555 mg/cu m) for the next 5 weeks, and 750 ppm (2,330 mg/cu m) for the final 17 weeks. ... Carbon disulfide in the exhaled breath averaged 1.4 ppm (4.3 mg/cu m) when the exposure concn was 500 ppm (1,555 mg/cu m) and rose to 3.1 ppm (9.6 mg/cu m) when the exposure concentration was 750 ppm (2,330 mg/cu m). No carbon disulfide was detected in the exhaled breath of rabbits exposed at 250 ppm (775 mg/cu m).
[Cohen AE, et al; Am Ind Hyg Assoc J 20: 303-23 (1959) as cited in NIOSH; Criteria Document: Carbon Disulfide p.92-4 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Absorption, Distribution & Excretion :

The relationship between carbon disulfide disposition and development of carbon disulfide neurotoxicity is reviewed. Animal studies have indicated that approximately 8 to 30% of the carbon disulfide retained after inhalation exposure is excreted by the lung; amounts less than 0.5% of retained carbon disulfide are excreted by the kidney. Free carbon disulfide reaches steady state concentrations in various tissues within 4 to 5 hours after initiation of exposure.
[Bus JS; Neurotoxicology 6 (4): 73-80 (1985)]**PEER REVIEWED**

Absorption, Distribution & Excretion :

Despite considerable variation between individuals, absorption seems to be proportional to the concentration of carbon disulfide in the inhaled air, and equilibrium between the carbon disulfide content of inhaled and exhaled air is reached in 1-2 hours. At this point, the percentage retained is about 40-50%, and carbon disulfide is distributed in the organism by the bloodstream, where twice as much is taken up by the erythrocytes as by the plasma. As carbon disulfide is readily soluble in fats and lipids, and binds to amino acids and proteins, it disappears rapidly from the bloodstream and has a high affinity for all tissues and organs. The order of affinity for different organs has not been established in man. Between 10 and 30% of absorbed carbon disulfide is exhaled, less than 1% is excreted in the urine ... .
[WHO; Environ Health Criteria: Carbon Disulfide p.2 (1979)]**PEER REVIEWED**

Environmental Water Concentrations :

SURFACE WATER: The mean concn of carbon disulfide in the open waters of the Atlantic Ocean and the Atlantic Ocean of Ireland are 0.52 and 0.78 parts per trillion, respectively(1). The mean concn in stagnant bay water was 5.4 parts per trillion(1). Water samples from 82 stations in Lake Ontario and 17 in the lower Niagara River were analyzed for volatile organics(2). Two river samples contained 25 parts per trillion of carbon disulfide while the other station contained <20 parts per trillion, the detection limit(2). Eleven lake samples contained quantifiable amounts of carbon disulfide whose median and max concn was 400 and 3900 parts per trillion, respectively(2). Half of the other samples contained trace quantities of the chemical and the other stations contained <80 parts per trillion, the detection limit(2). Carbon disulfide was prominent in Toronto Harbor, with lower levels in Hamilton Harbor and Oak Orchard Creek(2). In another study, carbon disulfide was detected, but not quantified, in the central basin of Lake Erie, the Niagara River, and open waters of Lake Ontario; it was absent from the western basin of Lake Ontario(3).
[(1) Lovelock JE; Nature 248: 625-6 (1974) (2) Kaiser KLE et al; J Great Lakes Res 9: 212-23 (1983) (3) Great Lakes Water Quality Board; An Inventory Of Chemical Substances Identified In The Great Lakes Ecosystem Volume 1 Windsor Ontario, Canada p. 195 (1983)]**PEER REVIEWED**

Effluent Concentrations :

In a comprehensive survey of wastewater from 4000 industrial and publicly owned treatment works (POTWs) sponsored by the Effluent Guidelines Division of the US EPA, carbon disulfide was identified in discharges from the following industrial category (frequency of occurrence; median concn in ppb): leather tanning (1; 7.5), paint and ink (4; 1078.6), organics and plastics (30; 1654.3), plastics and synthetics (4; 7075.4), pulp and paper (2; 215.6), pesticides manufacture (1; 88.8), publicly owned treatment works (11; 45.8)(1). The highest effluent concn was 18,943 ppb in the plastics and synthetics industry(1). In a survey of 63 industrial waste water effluents, carbon disulfide was identified in 8 samples, 6 of which were <10 ppb and 2 between 10 and 100 ppb(2). The concn of carbon disulfide in offgas from two oil shale retorting processes were 24 ppm and 13 ppm(3). Carbon disulfide was found in both the influent and effluent of a large community septic tank(4). The combined concn of carbon disulfide and dichloromethane in the effluent, which was 10 ppb, was much higher than that in the influent and reflected the presence of anaerobic processes in the sewer line or septic tank(4).
[(1) Shackelford WM et al; Analyt Chim Acta 146: 15-27 (1983) (2) Perry DL et al; Identification Of Organic Compound In Industrial Effluent Discharges USEPA-600/4-79-016 (NTIS PB-294784) p. 230 (1979) (3) Sklarew DS et al; Environ Sci Techol 18: 592-600 (1984) (4) Dewalle FB et al; Determination Of Toxic Chemicals In Effluent From Household Septic Tanks USEPA-600/S2-75-050 p. 4 (1985)]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

/A study was conducted to determine the/ biochemical changes due to carbon disulfide in 11 male New Zealand white rabbits. The rabbits were exposed to carbon disulfide by inhalation for 6 hours/day, 5 days/week, for up to 38 weeks. Concentrations of carbon disulfide were 250 ppm (775 mg/cu m) during the first 16 weeks, 500 ppm (1,555 mg/cu m) for the next 5 weeks, and 750 ppm (2,330 mg/cu m) for the final 17 weeks. ... Total serum cholesterol increased in exposed rabbits when the carbon disulfide concentration was increased to 750 ppm (2,330 mg/cu m) and returned to normal after cessation of exposure. ... Increased urinary and fecal excretion of zinc by the exposed rabbits and a gradual decrease in the mean concentration of zinc in the blood serum /was noted/ during the study.
[Cohen AE, et al; Am Ind Hyg Assoc J 20: 303-23 (1959) as cited in NIOSH; Criteria Document: Carbon Disulfide p.92-4 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Absorption, Distribution & Excretion :

Skin absorption of carbon disulfide vapor in male albino rabbits was studied. ... After 3 hours of dermal exposure to carbon disulfide at 1,550 ppm (4820 mg/cu m), the exhaled air of the rabbit contained 2.5 ppm (7.8 mg/cu m) of the cmpd; 0.25 ppm (0.78 mg/cu m) could still be detected 1.5 hours after cessation of the 3-hour exposure. Exposure of one rabbit to carbon disulfide at 1,500 ppm (4665 mg/cu m), 3 hours/day for 8 consecutive days revealed that the concn of carbon disulfide in the exhaled breath increased with the length of exposure. ...
[Cohen AE et al; AMA Arch Ind Health 17: 164-9 (1958) as cited in NIOSH; Criteria Document: Carbon Disulfide p.91 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Interactions :

The effects of isopropyl alcohol on hepatotoxicity of carbon disulfide was examined in rat. Metabolism of carbon disulfide was previously reported to involve two forms of cytochrome p450, a high affinity/low capacity isoenzyme and a low affinity/high capacity isoenzyme. An 18 hr pretreatment with isopropyl alcohol, resulted in a significantly greater decr in cytochrome p450-dependent aniline hydroxylase activity than without pretreatment. In addition, plasma transaminase activity (another hepatotoxic parameter of carbon disulfide) incr. Shorter pretreatment periods (from 2 hr to simultaneous admin) with the alcohol had no effect on the CS2 induced damage to p450 while markedly reducing the damage assessed by plasma transaminases of isopropyl alcohol. The results support the hypothesis that the hepatotoxic effect of carbon disulfide results from its metabolism, which is induced after an 18 hr exposure to isopropyl alcohol but which is inhibited when the liver is exposed to both the alcohol and CS2 at the same time.
[Rubin RJ, Kroll R; Adv Exp Med Biol 197(3): 237-42 (1986)]**PEER REVIEWED**

Volatilization from Water/Soil :

The Henry's Law constant for carbon disulfide is 1.44X10-2 atm-cu m/mole at 24 deg C(1). This Henry's Law constant indicates that carbon disulfide is expected to volatilize rapidly from water surfaces(2,SRC). Based on this Henry's Law constant, the estimated volatilization half-life from a model river (1 m deep, flowing 1 m/sec, wind velocity of 3 m/sec) is approximately 2.6 hours(2,SRC). The estimated volatilization half-life from a model lake (1 m deep, flowing 0.05 m/sec, wind velocity of 0.5 m/sec) is approximately 3.5 days(2,SRC). Carbon disulfide's Henry's Law constant(1) indicates that volatilization from moist soil surfaces is expected to occur(SRC). The Henry's Law constant in seawater at 24 deg C is 1.61X10-2 atm-cu m/mole(1). The Henry's Law constant increases by a factor of four between 0.5 deg C and 32 deg C(1). The potential for volatilization of carbon disulfide from dry soil surfaces may exist(SRC) based upon the vapor pressure of 359 mm Hg(3).
[(1) Elliot S; Atmos Environ 23: 1977-80 (1989) (2) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington DC: Amer Chem Soc pp. 15-1 to 15-29 (1990) (3) Yaws CL; Handbook of Vapor Pressure, Vol 1, Houston,TX: Gulf Pub Co (1994)]**PEER REVIEWED**

Atmospheric Concentrations :

RURAL/REMOTE: Levels of carbon disulfide in 61 air samples taken over the course of 2 days at Wallops Island, VA ranged from 29 to 84 parts per trillion; median 41 parts per trillion(1). At Harwell, England concns ranged from 70 to 370 parts per trillion; avg 190 parts per trillion(3). Concns of carbon disulfide varied with altitude, measuring: 115 parts per trillion mean at 6.1 km altitude, 23 parts per trillion mean at 7.3 km altitude, and 26 parts per trillion at 7.3 to 7.9 km altitude(1). The variation with altitude has been ascribed to the updrafting of boundary layer air by cumulus and cumulonimbus activity to the upper troposphere(1). URBAN/SUBURBAN: Mean, minimum, and maximum carbon disulfide concns in Philadelphia measured over a 40 day period (88 samples) were 65, 25, and 340 parts per trillion(2). It was detected, but not quantified, in air in Leningrad(4). Carbon disulfide was detected in air from three U.S. urban/suburban locations between 1979 to 1982 at a mean concn of 0.3 ug/cu m; range 0.05 to 1.07 ug/cu m(5).
[(1) Carroll MA; J Geophys Res 90: 10483-6 (1985) (2) Brodzingsky R, Singh HB; Volatile Organic Chemicals In The Atmospheric: An Assessment of Available Data Menlo Park,CA: Atmospheric Science Center SRI International Contract 68-02-3452 pp. 198 (1982) (3) Sandalla FJ, Penkett SA; Atmos Environ 11: 197-9 (1977) (4) Ioffe BV et al; J Chromatogr 142: 787-95 (1977) (5) Kelly TJ et al; Ambient concn Summaries for Clean Air Act. Title III. Hazardous Air Pollutants. U.S. EPA Contract No. 68-D80082, USEPA/600/R-94/090, Final Report Research Triangle Park (1993)]**PEER REVIEWED**

Probable Routes of Human Exposure :

NIOSH (NOES Survey 1981-1983) has statistically estimated that 44,441 workers, including 4,882 females, are exposed to carbon disulfide in the USA(1). Since the NOES survey excludes exposure to trade name chemicals which may contain the chemical, occupational exposure may be considerably higher(SRC). Occupational exposure to carbon disulfide may occur through inhalation and dermal contact with this compound at workplaces where carbon disulfide is produced or used(SRC). The general population may be exposed to carbon disulfide via inhalation of ambient air or ingestion of fruits, vegetables, and other food products containing this compound(SRC).
[(1) NIOSH; National Occupational Health Survey (NOHS) (1985)]**PEER REVIEWED**

Clinical Laboratory Methods :

The use of blood, exhaled air and urine as biological monitors of exposure to carbon disulfide was studied in England. A metabolite of carbon disulfide, 2-thiothiazolidine-4-carboxylic acid was identified in urine through high performance liquid chromatography. The head space analysis used was a sulfur specific detector to determine acid labile carbon-disulfide in blood. End expired breath samples were obtained through forced exhalation and carbon disulfide was determined by a quadrupole mass spectrometer. A general trend suggested increased uptake with increasing exposure. Reproducibility was difficult to achieve.
[Campbell L et al; Amer J Indust Med 8 (2): 143-53 (1985)]**PEER REVIEWED**

Methods of Manufacturing :

... Acetylene and sulfur vapor. Carbon disulfide and benzene are obtained from sulfur and ... ethylene, above 1000 deg C. ... For reaction of hydrogen sulfide and carbon at 900 deg C, a 70% conversion to carbon disulfide has been claimed. Methane and sulfur dioxide form carbon disulfide at elevated temp in the presence of suitable catalysts, such as lead sulfide on pumice activated with hydrogen chloride, giving an 84% yield at 850 deg C. ... /Using/ anthracite ... instead of methane, a nearly quantitative conversion was obtained at 900-1000 deg C.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present.V5 65 (1993)]**PEER REVIEWED**

Human Toxicity Excerpts :

350 artificial-fiber plant workers were examined to determine if there were changes in the oral cavity associated with exposure to carbon disulfide. The workers had been exposed to carbon disulfide at concentrations of 0.02-0.065 mg/liter (6-21 ppm) and to hydrogen sulfide at 0.002-0.006 mg/liter (1-4 ppm) during the preceding 6 years ... . The group exposed to carbon disulfide for less than 5 years had significantly lower pH values for both the mucous membrane and the saliva than did the controls (5.28 versus 6.09 and 5.30 versus 6.29, respectively). Workers exposed for longer periods did not show this difference. Based on an index of periodontic disturbances, the frequency of pathologic changes in the periodontium of the exposed workers was significantly higher than that of the controls. The intensity of these changes increased with length of exposure, although the levels of significance did not. ...
[Gondzik W et al; Med Pracy 20: 78-83 (1969) as cited in NIOSH; Criteria Document: Carbon Disulfide p.81 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

/An investigation was conducted to determine the effects of carbon disulfide exposure on/ female viscose rayon workers in three different departments for possible effects of /the compound/ on ovarian function and menstruation. The study included 500 workers in the spinning shop, where carbon disulfide concentrations sometimes exceeded 20 mg/cu m (6 ppm) and hydrogen sulfide concentrations reportedly never exceeded 10 mg/cu m (7 ppm); 209 workers in the trimming department, where the concentration of neither carbon disulfide nor hydrogen sulfide exceeded 10 mg/cu m (3 ppm); and 429 workers in the rewinding-sorting department (controls), not exposed to either substance. Durations of menstrual flow of more than 5 days occurred in 17.8% of the spinners, 10.5% of the trimmers, and 5.1% of the controls (p< 0.0001). Workers in the spinning shop experienced irregular menstruation significantly more frequently than the controls (7.6% and 1.6%, respectively; p< 0.0001). The frequency of irregular menses increased with longer occupational exposure. Heavy menstrual flow occurred in 12.5% of the spinners, 11% of the trimmers, and 2.3% of the controls (p< 0.001); painful menstruation was also significantly more common in exposed workers (36% and 38%) than controls (17%).
[Vasilyeva IA; Gig Sanit 7: 24-7 (1973) as cited NIOSH; Criteria Document: Carbon Disulfide p.56 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

Cases of carbon disulfide poisoning were reported. A 24 year old male, employed in the curing room of an India rubber factory, was exposed to carbon disulfide vapors for 8 months. He was admitted to the hospital complaining of loss of sensation in the limbs, weakness, restlessness, insomnia, memory loss, weight loss, and atrophy of arm and leg muscles. The subject had a high stepping gait, and had difficulty standing erect. He had no ability to produce dorsal flexion of the ankle, extension of the big toe, or inversion of the foot. After 2.5 months, some traces of paralysis were still seen, particularly in the dorsal flexions of the foot. Another subject, a 36 year old male, had been exposed to carbon disulfide fumes for 9 months in the curing room of an India rubber factory. He complained of weakness, visual effects, deafness in one ear, pains and cramping in the lower extremities, headache, insomnia, apathy, and memory loss. His paralysis was very similar to that described for the other subject. After 1 month in the hospital, the subject was much improved, although the muscles that produced dorsal flexion of the feet were still comparatively paralyzed.
[Ross J; Medical Chronicle 5 (28): 257-69 (1987)]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

Effects of carbon disulfide on testicular tissues of rats were studied. Three experiments were conducted using 85 mongrel rats, 2-5 months old and weighing 200-260 g. In the first experiment, 12 rats were injected ip every second day for 60 days with 12.5 mg/kg of distilled carbon disulfide dissolved in peanut oil; 5 were given pure peanut oil; and 5 were untreated. In the second experiment, 15 animals were given ip doses of 25.0 mg/kg every other day for 60 days; 10 rats were given pure peanut oil; and 9 were untreated. In the third experiment, 10 were given 25.0 mg/kg ip every other day for 120 days; 10 were injected with peanut oil; and 9 were untreated. ... The testicles of rats from exposed and control groups had similar histologic and histochemical patterns. However, exposed rats had thickened vascular walls, blood-cell-engorged vessels, disorganized seminiferous epithelium, and decreased numbers of spermatozoa. Rats injected with carbon disulfide for a 120-day period, however, showed marked testicular damage. Advanced regressive lesions involving all parts of the testicles of the usually round and smooth tubular basement membrane. Spermatogonia were few and sometimes nonexistent in the seminiferous tubules, and spermatogenesis was absent. Leydig cells showed degeneration and atrophy.
[Gondzik M; Pol Med J 10: 133-9 (1971) as cited in NIOSH; Criteria Document: Carbon Disulfide p.88 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Absorption, Distribution & Excretion :

LARGE CONCN OF BOTH FREE & BOUND CARBON DISULFIDE ARE FOUND IN BRAIN (GUINEA PIG STUDIES) & PERIPHERAL NERVES (RAT STUDIES) OF EXPOSED ANIMALS. RATIO OF BOUND TO FREE CARBON DISULFIDE IN BRAIN IS 3:1. BLOOD & FATTY TISSUES CONTAIN MAINLY BOUND CARBON DISULFIDE, WHILE LIVER CONTAINS MAINLY FREE.
[National Research Council. Drinking Water & Health Volume 1. Washington, DC: National Academy Press, 1977.701]**PEER REVIEWED**

Interactions :

Inhibition of drug metabolism in 19 healthy men experimentally exposed to carbon disulfide was studied. The men, 21-40 years old, were exposed for 6 consecutive hours in an inhalation chamber to carbon disulfide at 10, 20, 40, or 80 ppm (31, 62, 124, or 248 mg/cu m) ... . In the first of three experiments, each subject received 7 mg/kg of amidopyrine orally just prior to chamber exposure ... . Urine samples collected 3, 6, 9, 12, 16, 24, and 33 hours after the beginning of exposure were analyzed for metabolites of amidopyrine, 4-aminoantipyrine, and acetyl-4-aminoantipyrine. ... In the single 6 hour exposures, concentrations of 10 ppm (31 mg/cu m) caused no appreciable reduction in urinary excretion of acetyl-4-aminoantipyrine but caused significant reductions in free 4-aminoantipyrine and total 4-aminoantipyrine. At carbon disulfide concentrations of 20, 40, and 80 ppm (31, 124, 248 mg/cu m), reductions in free 4-aminoantipyrine, acetyl-4-aminoantipyrine, and total 4-aminoantipyrine were statistically significant. ...
[Mack T et al; Biochem Pharmacol 23: 607-14 (1974) as cited in NIOSH; Criteria Document: Carbon Disulfide p.34 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Environmental Fate :

TERRESTRIAL FATE: Based on a classification scheme(1), an estimated Koc value of 270(SRC), determined from a log Kow of 1.94(2) and a regression-derived equation(3), indicates that carbon disulfide is expected to have moderate mobility in soil(SRC). Volatilization of carbon disulfide from moist soil surfaces is expected to occur(SRC) given a Henry's Law constant of 1.44X10-2 atm-cu m/mole at 24 deg C(4). The potential for volatilization of carbon disulfide from dry soil surfaces may exist(SRC) based upon a vapor pressure of 359 mm Hg(5). Biodegradation data are insufficient to predict the importance of biodegradation in the environment(SRC).
[(1) Swann RL et al; Res Rev 85: 23 (1983) (2) Hansch C et al; Exploring QSAR. Hydrophobic, Electronic, and Steric Constants. ACS Prof Ref Book. Heller SR (consult ed) Washington,DC: Amer Chem Soc p 3 (1995) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington DC: Amer Chem Soc pp. 4-9 (1990) (4) Elliot S; Atmos Environ 23: 1977-80 (1989) (5) Yaws CL; Handbook of Vapor Pressure, Vol 1, Houston,TX: Gulf Pub Co. (1994)]**PEER REVIEWED**

Soil Adsorption/Mobility :

The Koc of carbon disulfide is estimated as approximately 270(SRC), using a log Kow of 1.94(1) and a regression-derived equation(2,SRC). According to a classification scheme(3), this estimated Koc value suggests that carbon disulfide is expected to have moderate mobility in soil(SRC). The avg adsorption of carbon disulfide after 10 minutes by 4 air-dried soils was 46% but only 12% by the same soils at 50% water-holding capacity(4). However, after 8 hr the rate of adsorption was greater by moist soil, but only when the soil was unsterilized(4). Further experiments suggest that this "adsorption" in moist soils is the result of microbial action(4).
[(1) Hansch C et al; Exploring QSAR. Hydrophobic, Electronic, and Steric Constants. ACS Prof Ref Book. Heller SR (consult ed) Washington,DC: Amer Chem Soc p 3 (1995) (2) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington,DC: Amer Chem Soc pp. 4-9 (1990) (3) Swann RL et al; Res Rev 85: 23 (1983) (4) Bremner JM, Banwart WL; Soil Biol Biochem 8: 79-83 (1976)]**PEER REVIEWED**

Atmospheric Concentrations :

SOURCE DOMINATED: Workplace concn of carbon disulfide in the viscose rayon industry ranged from <3 ppm to peaks exceeding 2,000 ppm(1). 12 of 36 air samples of carbon disulfide in the breathing zone of workers in the spinning and cutting rooms of a viscose rayon plant contained >20 ppm 8 hr TWA, the OSHA standard and 7 samples exceeded 100 ppm, the acceptable max peak concn(1). In a further study, 10-20 min breathing zone concn of 8 cutters ranged from <20 to >2,000 ppm and exceeded 100 ppm in more than half of the 196 samples taken(1). The concns for 6 workers in the spinning area were far lower. The overall TWA concn was 11.2 ppm with a range of 0.9 to 127 ppm(1). Shift TWA concns for the cutters ranged from 9.5 to 129 ppm for the cutters and 4.3 to 11.1 ppm for the spinners(1). Seven of the eight cutters were exposed to concn higher than the OSHA standard for an 8 hr day(1). Half of the general room samples exceed the 30 ppm ceiling limit(1). Extensive long term monitoring in a Finnish viscose rayon plant showed that concn levels of carbon disulfide that generally exceeded 40 ppm before 1950 have been dropping and had fallen below 5 ppm by 1972(1). Environmental concn in a US viscose rayon plant were reported to be between 10 to 15 ppm(1).
[(1) NIOSH; Criteria for a Recommended Standard, Occupational Exposure to Carbon Disulfide DHEW (NIOSH) Publ No 77-156 (1977)]**PEER REVIEWED**

Body Burden :

All 8 samples of mother's milk from 4 urban areas of the U.S. contained carbon disulfide(1). Carbon disulfide was qualitatively detected in samples of mother's milk from Bayonne, NJ, Jersey City, NJ, Pittsburgh, PA, and Baton Rouge, LA(2). Diurnal 2-thiothiazolidine-4-carboxylic acid, an indicator of exposure to carbon disulfide, excretion in urine samples collected from viscose production workers ranged from 3.4 to 41.5 mmol(3). Urinary concn of 2-thiothiazolidine-4-carboxylic acid (TTCA) of workers during the production of viscose rayon fibers ranged from not detected to 2.3 mg TTCA/g creatinine(4). Mean TTCA concns in urine of workers in the viscose industry were, mg/l (department): 1.96 (spool spinning), 5.06 (spinning of technical rayon), 6.55 (washing), 2.27 (post-treatment), and 1.14 (second aging)(5).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982) (2) Erickson, MD et al; Acquisition and Chemical Analysis of Mother's Milk for Selected Toxic Substances. USEPA-560/13-80-029. Washington,DC: USEPA, Off Pestic Toxic Subst (1980) (3) Riihimaki V et al; Am J Ind Med 2: 85-97 (1992) (4) Kitamura S et al; Int Arch Occup Environ Health 65: S177-80 (1993) (5) Drexler H et al; Int Arch Occup Environ Health 65: 359-65 (1994)]**PEER REVIEWED**

Corrosivity :

Carbon disulfide is normally stored and handled in mild steel equipment. Copper and copper alloys are attacked by carbon disulfide and must be avoided.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present.V5 65 (1993)]**PEER REVIEWED**

Hazardous Reactivities & Incompatibilities :

Carbon disulfide vapor, alone or mixed with nitrogen, did not decompose explosively in the range 0.4-2.1 bar/88-142 deg C when initialized with high energy sparks or a hot wire at 700-900 deg C. The endothermic sulfide will ... decompose explosively to its elements with mercury fulminate initiation. A screening jacket filled with carbon disulfide was used to surround the reaction tube used in flash photolysis experiments. When the quartz lamp was discharged, some vapor of the disulfide which had leaked out, ignited in the radiation flash and exploded.
[Bretherick, L. Handbook of Reactive Chemical Hazards. 4th ed. Boston, MA: Butterworth-Heinemann Ltd., 1990196]**PEER REVIEWED**

Disposal Methods :

Carbon disulfide is a waste chemical stream constituent which may be subjected to ultimate disposal by controlled incineration. A sulfur dioxide scrubber is necessary when combusting significant quantities of carbon disulfide.
[USEPA; Engineering Handbook for Hazardous Waste Incineration p.2-5 (1981) EPA 68-03-3025]**PEER REVIEWED**

Disposal Methods :

Adsorption: The CS2 /carbon disulfide/ adsorption employs activated coal which adsorbs it from the mixture with hydrogen sulfide in the absence of free oxygen. An industrial installation with the capacity to regenerate as much as 80-90% of carbon disulfide contained in the gas-air mixture has been developed.
[United Nations. Treatment and Disposal Methods for Waste Chemicals (IRPTC File). Data Profile Series No. 5. Geneva, Switzerland: United Nations Environmental Programme, Dec. 1985.131]**PEER REVIEWED**

Medical Surveillance :

Other Tissues: The assessment of carbon disulfide exposure can also be accomplished through measurement of carbon disulfide in breath and milk samples. Air levels may yield some information about recent, short term exposure, but correlation with actual exposure levels has been poor. Milk analysis may be useful as a qualitative indicator of exposure only.
[Ryan, R.P., C.E. Terry (eds.). Toxicology Desk Reference 4th ed. Volumes 1-3. Taylor & Francis, washington, D.C. 1997.624]**PEER REVIEWED**

Human Toxicity Excerpts :

Five hundred synthetic fiber workers who had been exposed to carbon disulfide at concentrations reportedly not exceeding 0.01 mg/liter (3 ppm) were studied. Workers were 18-60 years old and had been exposed for periods of 0.5-30 years. Those exposed for short periods of time (usually less than 5 years) generally had mild visual disturbances such as conjuntival inflammations, temporary corneal opacities, and disturbed color vision. Prolonged exposure to carbon disulfide was reported to have caused irreversible vascular effects and inflammatory degenerative changes in the retina.
[Szymankowa G; Klin Oczna 38: 41-4 (1968)]**PEER REVIEWED**

Human Toxicity Excerpts :

Male viscose rayon workers, diagnosed as chronically poisoned by carbon disulfide were studied. ... Concentrations at which the viscose rayon workers were exposed were 10-30 ppm (31-93 mg/cu m) in the 1960's, 20-40 ppm (62-124 mg/cu m) in the 1950's, and higher than 40 ppm (124 mg/cu m) prior to 1950. The most significant differences between the poisoned and control groups were the prevalence of general fatigue, insomnia, paresthesia, and headaches in the exposed workers (p< 0.001 for all four symptoms). Psychologic testing revealed mild intellectual impairment, reduction of sensorimotor speed, and impaired psychomotor ability. The psychologic disturbances were said to correlate well with duration of exposure, ie, patients with shorter carbon disulfide histories generally had milder disturbances.
[Seppalainen AM et al; Work Environ Health 9: 71-5 (1972) as cited in NIOSH; Criteria Document: Carbon Disulfide p.64 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

Pregnancy data for 380 women employed in the viscose industry /were analyzed/ to determine the effects of carbon disulfide on pregnancy. The exposed group included 189 women who, before and during pregnancy, were exposed to carbon disulfide at concentrations reported to be 2.7 times the Soviet permissible limit of 10 mg/cu m (3 ppm). ... Several pregnancy complications were recorded, and comparisons were made between exposed and control women. The rate of threatened pregnancy terminations in the exposed group was 25.9/100 pregnant women versus 13.1/100 pregnant women in the controls (p< 0.05). The difference was still significant after adjustment for the differences in age and job longevity. Threatened pregnancy terminations occurred more frequently in the exposed women than in the controls, 12.5% versus 9.4% in the 20- to 24-year-old age group and 35.4% versus 13.6% in the 25- to 29-year-old age group. Spontaneous abortions occurred in 14.3% of the exposed women and 6.8% of the controls (p< 0.05).
[Petrov MV; Pediatr Akush Ginekol 3: 50-2 (1969) as cited in NIOSH; Criteria Document: Carbon Disulfide p.58 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

Thirty workers of a viscose rayon industry had a complete eye examination in 1979 including visual acuity, perimetry, color vision testing, fluorescein angiography, ERG and EOG, for possible signs of chronic carbon disulfide poisoning. Fundus anomalies and abnormal electrooculogram's and electroretinogram's were found. Twenty-nine of these thirty workers were reexamined in 1983. A number of them were no longer exposed to carbon disulfide for a period varying between 1 and 43 months. The fundus signs (pigmentary changes and vascular lesions) increased in frequency, even if the patient was no longer exposed.
[De Rouck A, et al; Int Ophthalmol 9 (1): 17-27 (1986)]**PEER REVIEWED**

Human Toxicity Excerpts :

Carbon disulfide is a potent nerve toxin; it also may accelerate coronary artery disease. Peripheral neuropathies, cranial nerve dysfunction, and neuropsychiatric changes are present in over 70% of chronic carbon sulfide victims. Impaired psychomotor function ... and higher cortical function ... as well as neurasthenic symptoms ... characterize the neurologic illness associated with excessive carbon disulfide exposures. These neuropsychiatric symptoms may be irreversible. Chronic long-term exposures (eg, in rayon workers) may result in elevated blood cholesterol, retinopathy ... peripheral neuropathy, decreased glucose tolerance, reduced serum thyroxine levels, and parkinsonism. Increases in atherosclerosis, coronary artery disease, deaths, suicide rates, personality changes, and hypertensive disease have been suggested, but not confirmed, by epidemiological studies.
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988.819]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

Female albino rats were exposed to carbon disulfide vapor to study its effects on the course and duration of pregnancy. The animals, in groups of 12-20, were exposed to carbon disulfide at a concentration of 2,000 mg/cu m (642 ppm) for 2 hours/day during the entire pregnancy. Two identical series of tests were performed on rats. In the first experiment, 16.8% pre-implantation embryonic mortality occurred in the 12 exposed animals and 3.3% in the 12 controls (P< 0.05). In the second experiment, the pre-implantation mortality rate was 22.6% in 12 exposed rats and 6.5% in 14 controls (p< 0.05). The reproductive success of each exposed group was lower than that of its control group in both experiments (6.8 versus 9.7 fetuses per rat, p< 0.05, and 8.0 versus 9.3 fetuses per rat). There were seven post-implantation deaths in the fetuses of exposed rats and none in those of the controls. There were no significant differences between experimental and control rats in the mean corpus luteum counts or in mean fetal weights.
[Yaroslavskiy VK; Bull Eksp Biol Med 68: 88-91 (1969) as cited in NIOSH; Criteria Document: Carbon Disulfide p.89 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

Female Wistar rats were exposed to 100 or 600 ppm of carbon disulfide for 6 hr/day, 5 days/wk, for 12 wk to determine the effects on tissue Vitamin B6 concentrations. Liver, kidney, and brain tissue were assayed at the end of 12 wk (12 rats/group and 12 controls exposed to fresh air) for five forms of B6: pyridoxine, pyroxidal, pyridoxamine, pyridoxal phosphate, and pyridoxamine phosphate. During the experiment, urine was assayed for 4-pyridoxic acid on day 5 of wk 2, 4, 6, 10, and 12. By wk eight there were significant differences (p< 0.01) in the body weights of the 3 groups; 600 ppm, 208 + or - 7.5 g; 100 ppm, 216 + or - 9.2 g; and control, 221 + or - 5.2 g. Excretion of 4-pyridoxic acid throughout the experiment after exposure to 100 ppm was essentially the same as the control, but excretion of 4-pyridoxic acid after exposure to 600 ppm was significantly decreased (p< 0.01) during the first 4 wk of exposure and was continuous (p< 0.05) for the 12 wk. Pyridoxine was not detected in liver, kidney, or brain tissue and pyridoxamine was detected only in liver tissue. Even exposure to 600 ppm, carbon disulfide had no significant effect on the content of the forms of vitamin B6 in any of the tissues examined.
[Okayama A et al; Arch Toxicol 60 (6): 450-3 (1987)]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

... CARBON DISULFIDE IS ... RECOGNIZED AS INHIBITOR OF BRAIN MONOAMINE OXIDASE ... MONOAMINE OXIDASE ALSO CONTAINS COPPER AND UTILIZES PYRODOXYLPHOSPHATE (A FORM OF VITAMIN B6) AS A COENZYME. SINCE CARBON DISULFIDE CAN REACT WITH PYRIDOXAMINE TO FORM PYRIDOXAMINEDITHIOCARBAMIC ACID (WHICH IN TURN CAN BE OXIDIZED BY IODINE IN VITRO TO AN ANALOGUE OF DISULFIRAM), TWO POSSIBLE MECHANISMS EXIST FOR INHIBITION OF MONOAMINE OXIDASE.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-91]**PEER REVIEWED**

Absorption, Distribution & Excretion :

ABSORPTION OCCURS THROUGH ALL PORTALS INCLUDING THE INTACT SKIN. ... CARBON DISULFIDE VAPOR IS RAPIDLY ABSORBED WHEN INHALED; AN APPROXIMATE EQUILIBRIUM BETWEEN BLOOD AND INHALED VAPOR IS REACHED IN 1-2 HOURS. ... SOME ABSORBED CARBON DISULFIDE IS EXCRETED IN EXPIRED AIR ... TRACES HAVE BEEN FOUND IN THE BLOOD 80 HR AFTER TERMINATION OF EXPOSURE, ABOUT 70% OF AN INHALED DOSE IS EXCRETED OR METABOLIZED WITHIN A FEW HOURS. THE REMAINING 30% IS SLOWLY EXCRETED IN THE URINE AS SUCH OR AS METABOLITES.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-90]**PEER REVIEWED**

Mechanism of Action :

Chelation of copper-containing enzyme by the reaction products of carbon disulfide and biological amines has been observed and has been proposed as one of the mechanisms by which carbon disulfide induces neurotoxicity.
[Mckenna MJ, Distefano V; Pharmacol Exp Ther 202: 253-66 (1977)]**PEER REVIEWED**

Environmental Fate :

AQUATIC FATE: Based on a classification scheme(1), an estimated Koc value of 270(SRC), determined from a log Kow of 1.94(2) and a regression-derived equation(3), indicates that some adsorption of carbon disulfide to suspended solids and sediment in the water column is expected(SRC). Carbon disulfide is expected to volatilize from water surfaces(3,SRC) based on a Henry's Law constant of 1.44X10-2 atm-cu m/mole at 24 deg C(4). Estimated volatilization half-lives for a model river and model lake are 2.6 hours and 3.5 days, respectively(3,SRC). According to a classification scheme(5), BCFs of <6.1 and <60 in carp at 50 and 5 ug/l, respectively(6) suggest bioconcentration in aquatic organisms is low to moderate(SRC). Biodegradation data are insufficient to predict the importance of biodegradation in the environment(SRC).
[(1) Swann RL et al; Res Rev 85: 23 (1983) (2) Hansch C et al; Exploring QSAR. Hydrophobic, Electronic, and Steric Constants. ACS Prof Ref Book. Heller SR (consult ed) Washington,DC: Amer Chem Soc p 3 (1995) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods. Washington,DC: Amer Chem Soc pp. 4-9, 5-4, 5-10, 15-1 to 15-29 (1990) (4) Elliot S; Atmos Environ 23: 1977-80 (1989) (5) Franke C et al; Chemosphere 29: 1501-14 (1994) (6) Chemicals Inspection and Testing Institute; Biodegradation and Bioaccumulation Data of Existing Chemicals Based on the CSCL Japan. Japan Chemical Industry Ecology-Toxicology and Information Center. ISBN #4-89074-101-1 p. 1-1 (1992)]**PEER REVIEWED**

Environmental Fate :

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), carbon disulfide, which has a vapor pressure of 359 mm Hg at 25 deg C(2), is expected to exist solely as a vapor in the ambient atmosphere. Vapor-phase carbon disulfide is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC); the half-life for this reaction in air is estimated to be 5.5 days(3,SRC).
[(1) Bidleman TF; Environ Sci Technol 22: 361-367 (1988) (2) Yaws CL; Handbook of Vapor Pressure, Vol 1, Houston,TX: Gulf Pub Co (1994) (3) Arnts RR et al; J Air Pollut Control Assoc 39: 453-60 (1989)]**PEER REVIEWED**

Environmental Biodegradation :

It has been demonstrated that the adsorption of carbon disulfide by moist unsterilized soil increases sharply after approximately 3 hr and the time for complete sorption of the gas decreases with repeated dosing(1). This behavior does not occur with air-dried or sterilized soil and has been ascribed to microbial utilization of the chemical(1). Carbon disulfide is oxidized by some heterotrophs(2).
[(1) Bremner JM, Banwart WL; Soil Biol Biochem 8: 79-83 (1976) (2) Kelly DR, Smith NA; Adv Microb Ecol 11: 345-85 (1990)]**PEER REVIEWED**

Environmental Water Concentrations :

DRINKING WATER: Drinking water samples from nine cities in the US and one rural well contained no carbon disulfide (no detection limit stated)(1). It was detected, but not quantified, in New Orleans(2), Miami, and Cincinnati drinking water(3). Water samples obtained from collapsible fabric tanks used for potable water storage for U.S. Army field exercises in 1982 and 1987 contained a maximum carbon disulfide concn of 33 ug/l(4).
[(1) Heikes DL; J Assoc Off Anal Chem 70: 215-77 (1987) (2) USEPA; New Orleans Area Water Supply Study Draft Analytical Report by the Lower Mississippi River Facility, Slidell, LA, Dallas, Texas: USEPA (1974) (3) USEPA; Preliminary Assessment of Suspected Carcinogens in Drinking Water Interim Report to Congress, June, 1975 Washington,DC (1975) (4) Hao OJ et al; J Amer Water Works Assoc: 92-98 (1992)]**PEER REVIEWED**

Effluent Concentrations :

Carbon disulfide was detected in air samples collected from blower exhausts, from an active composting operation, at a concn of 224 ug/cu m(1). Carbon disulfide was detected at a concn of 10 ppb in water collected from a monitoring well at the Ionia City Landfill, MI(2). It has been detected in leachate liquid from the Lipari Landfill, NJ at concns ranging from 5 to 42 ug/l(3).
[(1) VanDurme GP et al; Wat Environ Res 64: 19-27 (1992) (2) USEPA; Superfund Record of Decision (EPA Region 5): Ionia City Landfill, Ionia, MI (First Remedial Action, Sept 1989), U.S. EPA (Off Emerg Rem Response, Washington, DC USA). Report 1989, USEPA/ROD/RO5-89/105. NTIS PB90-178120 (1989) (3) USEPA; Superfund Record of Decision (EPA Region 7): Lipari Landfill, Mantau Township, Gloucester, NJ, July 11, 1988. Third Remedial Action, Report 1988, USEPA/ROD/RO2-88/074. NTIS PB89-219224 (1988)]**PEER REVIEWED**

Sediment/Soil Concentrations :

The mean concn of carbon disulfide in 5 samples of mud from the sea bottom was 29.5 parts per trillion(1). Concns in salt marshes ranged from 76 to 228 ng/l(2). Soil samples from the Lipari Landfill, NJ contained carbon disulfide at an avg concn of 220 ug/kg; maximum concn 1,300 ug/kg(3). It was also detected in soils from Chestnut Branch Marsh, located adjacent to the landfill, at concns ranging from 11 to 33 ug/kg(3).
[(1) Lovelock JE; Nature 248: 625-6 (1974) (2) Kelly DR, Smith NA; Adv Microb Ecol 11: 345-85 (1990) (3) USEPA; Superfund Record of Decision (EPA Region 7): Lipari Landfill, Mantau Township, Gloucester, NJ, July 11, 1988. Third Remedial Action, Report 1988, USEPA/ROD/RO2-88/074. NTIS PB89-219224 (1988)]**PEER REVIEWED**

Milk Concentrations :

All 8 samples of mother's milk from 4 urban areas of the U.S. contained carbon disulfide(1). Carbon disulfide was qualitatively detected in samples of mother's milk from Bayonne, NJ, Jersey City, NJ, Pittsburgh, PA, and Baton Rouge, LA(2).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982) (2) Erickson, MD et al; Acquisition and Chemical Analysis of Mother's Milk for Selected Toxic Substances. USEPA-560/13-80-029. Washington,DC: US EPA Off Pestic Toxic Subst (1980)]**PEER REVIEWED**

Probable Routes of Human Exposure :

Exposure to carbon disulfide is mostly occupational via inhalation and dermal contact with the vapor or dermal contact with the liquid. Inhalation is the principal route of absorption(1). While workers engaged in any process using carbon disulfide may be exposed to some degree, in practice, only workers in the viscose rayon industry are exposed to high concn(1).
[(1) World Health Organization; Environmental Health Criteria 10: Carbon Disulfide Geneva (1979)]**PEER REVIEWED**

Clinical Laboratory Methods :

Carbon disulfide in urine (treated with a solution of sodium azide, iodine and potassium iodide) using Iodine-Azide Test; concentrations of less than 20 ppm carbon disulfide in air were not detectable.
[Baselt RC; Biological Monitoring Methods for Industrial Chemicals p.65 (1980)]**PEER REVIEWED**

Methods of Manufacturing :

(1) Reaction of natural gas or petroleum fractions with sulfur. (2) From natural gas and hydrogen sulfide at very high temperature (plasma process). (3) By heating sulfur and charcoal and condensing the carbon disulfide vapors.
[Lewis, R.J., Sr (Ed.). Hawley's Condensed Chemical Dictionary. 12th ed. New York, NY: Van Nostrand Rheinhold Co., 1993220]**PEER REVIEWED**

Formulations/Preparations :

Modern plants generally produce carbon disulfide of about 99.99% purity.
[Ashford, R.D. Ashford's Dictionary of Industrial Chemicals. London, England: Wavelength Publications Ltd., 1994.474]**PEER REVIEWED**

Major Uses :

For Carbon disulfide (USEPA/OPP Pesticide Code: 016401) there are 0 labels match. /SRP: Not registered for current use in the U.S., but approved pesticide uses may change periodically and so federal, state and local authorities must be consulted for currently approved uses./
[U.S. Environmental Protection Agency/Office of Pesticide Program's Chemical Ingredients Database on Carbon disulfide (75-15-0). Available from the Database Query page at http://www.cdpr.ca.gov/docs/epa/epamenu.htm as of October 24, 2002.]**QC REVIEWED**

Major Uses :

Miscellaneous application include direct uses of carbon disulfide for the cold vulcanization of rubber, as a flame lubricant in cutting glass, and for generating petroleum catalysts ... .
[CHEMICAL PRODUCTS SYNOPSIS: Carbon Disulfide, (1985)]**PEER REVIEWED**

Consumption Patterns :

CHEMICAL PROFILE: Carbon disulfide. Carbon tetrachloride, 38%; rayon, 34%; rubber chemicals, 7%; cellophane and other regenerated cellulosics, 6%; agricultural chemicals, 5%; miscellaneous, 10%.
[Kavaler AR; Chemical Marketing Reporter 235 (1): 42 (1989)]**PEER REVIEWED**

Consumption Patterns :

CHEMICAL PROFILE: Carbon disulfide. Demand: 1988: 400 million lb; 1989: 390 million lb; 1993 /projected/: 325 million lb. (Includes imports, which totaled 2.8 million lb in 1987; exports are negligible.)
[Kavaler AR; Chemical Marketing Reporter 235 (1): 42 (1989)]**PEER REVIEWED**

Odor :

When pure, carbon disulfide has sweetish aromatic odor similar to that of chloroform.
[WHO; Environ Health Criteria: Carbon Disulfide p.1 (1979)]**PEER REVIEWED**

Corrosivity :

Liquid carbon disulfide will attack some forms of plastics, rubber, and coatings.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.2]**PEER REVIEWED**

Solubilities :

Solubility of water in carbon disulfide: 86 ppm at 10 deg C; 142 ppm at 25 deg C
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present.V5 55 (1993)]**PEER REVIEWED**

Other Chemical/Physical Properties :

DIPOLE MOMENT 0.0; HEAT OF FUSION 1.049 KCAL/MOLE; HEAT CAPACITY @ 24.3 DEG C: 18.17 CAL/MOLE/DEG; EBULLIOSCOPIC CONSTANT 2.35 DEG; DIELECTRIC CONSTANT 2.641 AT LOW FREQUENCIES; BURNS WITH BLUE FLAME TO CARBON DIOXIDE AND SULFUR DIOXIDE; AZEOTROPE WITH WATER BP 42.6 DEG C, CONTAINS 97.2% CARBON DISULFIDE
[Budavari, S. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 1996.296]**PEER REVIEWED**

Fire Potential :

Carbon disulfide vapor is explosive, igniting spontaneously on contact with sparks or at temperatures above 147 degrees C.
[Sullivan, J.B. Jr., G.R. Krieger (eds.). Hazardous Materials Toxicology-Clinical Principles of Environmental Health. Baltimore, MD: Williams and Wilkins, 1992.1061]**PEER REVIEWED**

Toxic Combustion Products :

Toxic gases and vapors (such as sulfur dioxide and carbon monoxide) may be released in a fire involving carbon disulfide.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.2]**PEER REVIEWED**

Hazardous Reactivities & Incompatibilities :

Disposal of 2 liters of the solvent into a rusted iron sewer caused an explosion. Initiation of the solvent-air mixture by rust was suspected. A hot gauze falling from a tripod into a lab sink containing some carbon disulfide initiated two explosions. ... The vapor or liquid has been known to ignite on contact with steam pipes, particularly if rusted. When a winchester of the solvent fell off a high shelf and broke behind a rusted steel cupboard, ignition occurred.
[Bretherick, L. Handbook of Reactive Chemical Hazards. 4th ed. Boston, MA: Butterworth-Heinemann Ltd., 1990197]**PEER REVIEWED**

Hazardous Reactivities & Incompatibilities :

The bis- or tris-complexes of phenylcopper with triphenylphosphine react violently and exothermically with carbon disulfide, even at 0 deg C.
[Bretherick, L. Handbook of Reactive Chemical Hazards. 4th ed. Boston, MA: Butterworth-Heinemann Ltd., 1990198]**PEER REVIEWED**

Hazardous Reactivities & Incompatibilities :

Carbon disulfide plus any of the azides produces violently explosive, extremely sensitive salts.
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997. 491-45]**QC REVIEWED**

Hazardous Reactivities & Incompatibilities :

Mixing carbon disulfide and ethylenediamine in a closed container caused the temperature and pressure to increase.
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997. 491-45]**QC REVIEWED**

Protective Equipment & Clothing :

If the use of respirators is necessary, the only respirators permitted are those that have been approved by the Mine Safety and Health Administration (formerly Mining Enforcement and Safety Administration) or by the National Institute for Occupational Safety and Health. ... Employees should be provided with and required to use impervious clothing, gloves, face shields (eight-inch minimum), and other appropriate protective clothing necessary to prevent skin contact. ... Employees should be provided with and required to use splash-proof safety goggles where liquid carbon disulfide may contact the eyes.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.3]**PEER REVIEWED**

Preventive Measures :

Areas suspected of high concn of carbon disulfide vapor should not be entered because of the explosion hazard.
[Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984.V4 750 (1978)]**PEER REVIEWED**

Preventive Measures :

CARBON DISULFIDE SHOULD NOT BE ALLOWED TO ENTER A CONFINED SPACE, SUCH AS A SEWER, BECAUSE OF THE POSSIBILITY OF AN EXPLOSION.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.4]**PEER REVIEWED**

Storage Conditions :

Protect containers against physical damage. Store in well detached and isolated places from other buildings, other materials and possible sources of ignition, preferably in a building of noncombustible, or better, constructed with floor level ventilation. Avoid direct sunlight. Tanks should be submerged in water or located over concrete basins containing water of sufficient compacity to hold all of the tank contents in addition to the water. Water or inert gas should be provided over the carbon disulfide in all tanks. No electrical installations or heating facilities should be permitted in or near storage area.
[ITII. Toxic and Hazardous Industrial Chemicals Safety Manual. Tokyo, Japan: The International Technical Information Institute, 1988.105]**PEER REVIEWED**

Cleanup Methods :

1. REMOVE ALL IGNITION SOURCES. 2. VENTILATE AREA OF SPILL OR LEAK. 3. FOR SMALL QUANTITIES, ABSORB ON PAPER TOWELS. EVAPORATE IN A SAFE PLACE (SUCH AS A FUME HOOD). ALLOW SUFFICIENT TIME FOR EVAPORATING VAPORS TO COMPLETELY CLEAR THE HOOD DUCTWORK. BURN THE PAPER IN A SUITABLE LOCATION AWAY FROM COMBUSTIBLE MATERIALS. LARGE QUANTITIES CAN BE RECLAIMED OR COLLECTED AND ATOMIZED IN A SUITABLE COMBUSTION CHAMBER EQUIPPED WITH AN APPROPRIATE EFFLUENT GAS CLEANING DEVICE. CARBON DISULFIDE SHOULD NOT BE ALLOWED TO ENTER A CONFINED SPACE, SUCH AS A SEWER, BECAUSE OF THE POSSIBILITY OF AN EXPLOSION.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981.4]**PEER REVIEWED**

Disposal Methods :

Reuse: This cmpd is a very flammable liquid which evaporates readily. It burns ... to carbon dioxide (harmless) and sulfur dioxide. ... The pure liquid presents an acute fire and explosion hazard. ... If quantity is large, carbon disulfide may be recovered by distillation and repackaged for use. Recommendable methods: Evaporation, adsorption, & incineration. Not recommendable method: Landfill. Peer-review: Care. Substance very easily ignited. Landfill is not recommendable due to the high flammability. Evaporate small amt only. (Peer-review conclusions of an IRPTC expert consultation (May 1985))
[United Nations. Treatment and Disposal Methods for Waste Chemicals (IRPTC File). Data Profile Series No. 5. Geneva, Switzerland: United Nations Environmental Programme, Dec. 1985.131]**PEER REVIEWED**

Antidote and Emergency Treatment :

For basic treatment: Establish a patent airway. Suction if necessary. Watch for signs of respiratory insufficiency and assist ventilations if necessary. Administer oxygen by nonrebreather mask at 10 to 15 L/min. Anticipate seizures and treat if necessary ... . Monitor for shock and treat if necessary ... . For eye contamination, flush eyes immediately with water. Irrigate each eye continuously with normal saline during transport ... . Do not use emetics. For ingestion, rinse mouth and administer 5 mL/kg up to 200 mL water for dilution if the patient can swallow, has a strong gag reflex, and does not drool. Administer activated charcoal ... . Cover skin burns with sterile dressings after decontamination ... . /Carbon disulfide and related compounds/
[Bronstein, A.C., P.L. Currance; Emergency Care for Hazardous Materials Exposure. 2nd ed. St. Louis, MO. Mosby Lifeline. 1994.397]**PEER REVIEWED**

Antidote and Emergency Treatment :

For advanced treatment: Consider orotracheal or nasotracheal intubation for airway control in the patient who is unconscious. Monitor cardiac rhythm and treat arrhythmias if necessary ... . Start an IV with lactated Ringer's TKO /SRP: To keep open, "minimal flow rate"/. Treat seizures with diazepam (Valium) ... . For hypotension with signs of hypovolemia, administer fluid cautiously. Watch for signs of fluid overload ... . Use proparacaine hydrochloride to assist eye irrigation ... . /Carbon disulfide and related compounds/
[Bronstein, A.C., P.L. Currance; Emergency Care for Hazardous Materials Exposure. 2nd ed. St. Louis, MO. Mosby Lifeline. 1994.398]**PEER REVIEWED**

Human Toxicity Excerpts :

WOMEN APPEAR TO BE MORE SENSITIVE THAN MEN TO THE NEUROTOXIC EFFECTS OF CARBON DISULFIDE. ... ABNORMALLY HIGH SERUM LEVELS OF CHOLESTEROL AND BETA-LIPOPROTEIN ... IN CHRONICALLY EXPOSED WORKERS ... WITH HIGH INCIDENCE OF HYPERTENSION AND ATHEROSCLEROSIS AND ... REDUCTION IN FIBRINOLYSIS ACTIVITY OF BLOOD SERUM. ... CHRONIC GASTRITIS WITH DYSPEPSIA ... .
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-92]**PEER REVIEWED**

Human Toxicity Excerpts :

IN A SURVEY OF RAYON FACTORIES WHERE CARBON DISULFIDE IN AIR WAS 37-56 MG/CU M, FEMALE SPINNERS SHOWED HIGH INCIDENCE OF MENSTRUAL DISTURBANCES & PREGNANCY TOXEMIA.
[CAI SX, BAO YS; IND HEALTH 19 (1): 15 (1981)]**PEER REVIEWED**

Human Toxicity Excerpts :

AT 10.2 UG/CU M MEDIUM, CARBON DISULFIDE INCREASED FREQUENCY OF SISTER CHROMATID EXCHANGES IN CULTURED HUMAN PERIPHERAL BLOOD LYMPHOCYTES BY APPROX 50%. LOWER CONCN HAD NO EFFECT.
[BASSENDOWSKA-KARSKA E; BROMATOL CHEM TOKSYKOL 14 (1): 115 (1981)]**PEER REVIEWED**

Human Toxicity Excerpts :

/A study was conducted to compare/ 118 male viscose rayon workers who had been exposed /to carbon disulfide/ for a mean of 15 years with 100 papermill workers (controls) for possible neurophysiologic differences. ... The greatest difference between exposed and control workers was found in the conduction velocities of the slower motor fibers in the ulnar nerve (39.8 versus 44.1 m/second, p< 0.0005) and the deep peroneal nerve (35.5 versus 38.2 m/second, p< 0.0005). Significant differences from normal were also found in the maximum motor conduction velocities of the posterior tibial nerve (40.5 versus 42.4 m/second, p< 0.0005) and deep peroneal nerve (45.9 versus 47.3 m/second, p< 0025). A conduction velocity was determined for each nerve tested such that 5% of the controls showed a conduction velocity below this value; each subject was then assigned a total conduction velocity score by counting one point for each nerve whose conduction velocity was below the limit for that nerve. The distribution of scores showed significantly slower conduction velocities in exposed workers. The lower conduction velocity scores /were regarded/ as an indication of increased polyneuropathy. The exposed group also had a larger number of abnormal EEG's (21 of 54) than did the controls (6 of 50); was significant at the 1% level.
[Seppalainen AM, Tolonen MT; Work Environ Health 11: 145-53 (1974) as cited in NIOSH; Criteria Document: Carbon Disulfide p.66 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

138 artificial silk workers whose exposure to carbon disulfide, averaging more than 10 years, had been at concentrations averaging between 20 and 42 mg/cu m (6-13 ppm) during the past 8 years, with peaks of 120-180 mg/cu m (39-58 ppm) /were examined/; earlier levels, believed to have been higher, were not documented. Atherosclerotic changes, as indicated by clinical, electrocardiographic, oscillometric, and optic fundi examination and by estimation of cholesterolemia, triglyceridemia, were found in 30.4% of the subjects and arterial hypertension in 23.2%; 14.5% of the workers showed both conditions. ...
[Gavrilescu N, Lilus R; Amsterdam, Excerpta Medic Foundation p.240 (1967) as cited in NIOSH; Criteria Document: Carbon Disulfide p.51 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

... Employees were often exposed to carbon disulfide 10-12 hours/day at concentrations of up to 2.50 mg/liter (800 ppm), although the mean concentrations ranged from 0.45 to 1.0 mg/liter (144-321 ppm). In the 100 workers examined, polyneuritic symptoms were observed in 88% of the patients. ...
[Vigliani EC; Br J Ind Med 11: 235-44 (1954) as cited in NIOSH; Criteria Document: Carbon Disulfide p.28 (1977) DHEW Pub NIOSH 77-156]**PEER REVIEWED**

Human Toxicity Excerpts :

WOMEN EMPLOYED IN RAYON TEXTILE JOBS & PAPER PRODUCTS JOBS HAD INCREASED RATE (P< 0.10) OF SPONTANEOUS ABORTIONS; THE WIVES OF MEN EMPLOYED IN TRANSPORT & COMMUNICATION, IN RAYON TEXTILE JOBS & IN CHEMICAL PROCESS JOBS ALSO HAD INCREASED RATE OF SPONTANEOUS ABORTIONS. NO EVIDENCE WAS FOUND THAT LEVEL OF CARBON DISULFIDE COULD BE ASSOC WITH RISK OF SPONTANEOUS ABORTIONS.
[HEMMINKI K, NIEMI ML; INT ARCH OCCUP ENVIRON HEALTH 51 (1): 55 (1982)]**PEER REVIEWED**

Human Toxicity Excerpts :

The spinners, the workers most heavily exposed to carbon disulfide, have a significantly higher mortality from all causes than the least exposed group. The excess mortality is largely accounted for by ischemic heart disease for which the spinners have a standard mortality ratio of 172. When mortality is related to an exposure score in the same group, both all cause (p< 0.01) and ischemic heart disease (p< 0.001) mortality increase with increasing exposure level. When this analysis is repeated covering all ages these trends become much less strong and only that for ischemic heart disease remains significant (p< 0.05). Over the age of 65 there is a tendency for mortality to decline with increasing exposure.
[Sweetnam RM et al; Br J Ind Med 44 (4): 220-7 (1987)]**PEER REVIEWED**

Human Toxicity Excerpts :

Effects of carbon disulfide in air: slight or none (160-230 ppm); slight symptoms after several hr (320-390 ppm); symptoms after 30 min (420-510 ppm); serious symptoms after 30 min (1150 ppm); dangerous to life after 30 min (3210-3850 ppm); fatal in 30 min (4815 ppm) /From table/
[Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984.V4 753 (1978)]**PEER REVIEWED**

Human Toxicity Excerpts :

Retinopathy seen in /workers exposed to carbon disulfide/ in Japan, ... consists of microaneurysns and small hemmorhages ... . In Finland positive findings were delayed peripapillary filling on fluoresein angiography, widening of retinal arterioles, and lower peak to ocular pulse wave.
[Amdur, M.O., J. Doull, C.D. Klaasen (eds). Casarett and Doull's Toxicology. 4th ed. New York, NY: Pergamon Press, 1991.550]**PEER REVIEWED**

Human Toxicity Excerpts :

Local contact results in erythema and pain since carbon disulfide is one of the most potent fat solvents. Prolonged contact produces vesiculation and chemical burns. Severe chemical burns of the cornea result from direct contact with the eyes.
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988.819]**PEER REVIEWED**

Human Toxicity Excerpts :

Adverse effects of carbon disulfide exposure on reproductive function...have been reported in exposed workers, with significantly lower sperm counts and more abnormal spermatozoa than in unexposed control subjects.
[Rom, W.N. (ed.). Environmental and Occupational Medicine. 2nd ed. Boston, MA: Little, Brown and Company, 1992.995]**PEER REVIEWED**

Human Toxicity Excerpts :

Of 27 patients that were acutely exposed to airborne carbon disulfide, 59% had a headache, 52% experienced nausea, 4% experienced vomiting, 40% felt a burning of the throat, lips, or skin, 59% experienced dizziness, 15% had shortness of breath or chest pain, and 7% experienced impotence.
[Sullivan, J.B. Jr., G.R. Krieger (eds.). Hazardous Materials Toxicology-Clinical Principles of Environmental Health. Baltimore, MD: Williams and Wilkins, 1992.1118]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

CARBON DISULFIDE WAS NOT MUTAGENIC TO SALMONELLA TYPHIMURIUM STRAINS TA98 & TA100 AT 300-1000 UMOL NOR TO ESCHERICHIA COLI STRAIN WP2 UVRA AT 20-600 UMOL WITH OR WITHOUT METABOLIC ACTIVATION, NOR IN DROSOPHILA MELANOGASTER AT 200-800 PPM.
[DONNER M ET AL; MUTAT RES 91 (3): 163 (1981)]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

PRENATAL EXPOSURE OF ALBINO RATS TO CARBON DISULFIDE AT 10 & 0.03 MG/CU M LED TO INHIBITION & RETARDATION OF DEVELOPMENT OF MIXED FUNCTION OXIDASE SYSTEM.
[NIKIFOROV B, TABACOVA S; ARCH TOXICOL (SUPPL) (4): 296 (1980)]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

Carbon disulfide exposure can induce kynureninase and lead to disorders of tryptophan metabolism.
[Amdur, M.O., J. Doull, C.D. Klaasen (eds). Casarett and Doull's Toxicology. 4th ed. New York, NY: Pergamon Press, 1991.711]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

...eight dogs were exposed to 400 ppm carbon disulfide in air 8 hr per day, 5 days per week, for 10-15 weeks. When removed from the chamber, the dogs were drowsy and staggered and stumbled as if drunk. They were very thirsty but did not eat for hours after leaving the chamber. Although they slept most of the time they were in the chamber, they were excited and noisy at night in their kennels. The dogs also developed many clinical and pathological signs analogous to those in workers: marked behavioral changes and toxic disease of nerve cells of the cortex of the brain were observed in all of them. Rigidity and tremor (parkinsonism) and choreatic movements were seen frequently, as was disease of the nerve cells of the basal ganglia. Motor weakness, flaccid paralysis, and nerve tenderness were the most frequent signs observed; the peripheral nerves showed axonal degeneration while the myelin sheath was well preserved. Cardiovascular changes included electrocardiographic abnormalitities, especially inversion of the T wave, retinal angiospasms, and artherosclerosis of the veins of the cortex of the brain.
[Hayes, W.J., Jr., E.R. Laws, Jr., (eds.). Handbook of Pesticide Toxicology. Volume 2. Classes of Pesticides. New York, NY: Academic Press, Inc., 1991.653]**PEER REVIEWED**

Non-Human Toxicity Excerpts :

In rats, carbon disulfide causes progressive testicular atrophy after repeated parenteral administration. The testes from these animals exhibit vasodilation, hemorrhage, and fluid exudation into the testicular parenchyma, suggesting a possible vascular origin for the atrophy. Testicular damage is not observed after inhalation exposure.
[Thomas, J.A., K.S. Korach, J.A. McLachlan. Endocrine Toxicology. New York, NY: Raven Press, Ltd., 1985.291]**PEER REVIEWED**

Human Toxicity Values :

Most acute carbon disulfide fatalities result from an ingestion of which 15 ml may be fatal to an adult.
[Ellenhorn, M.J. and D.G. Barceloux. Medical Toxicology - Diagnosis and Treatment of Human Poisoning. New York, NY: Elsevier Science Publishing Co., Inc. 1988.819]**QC REVIEWED**

National Toxicology Program Studies :

Carbon disulfide (CS), ... was evaluated for toxic and teratogenic effects in timed-pregnant CD rats. CS (0, 100, 200, 400 and 600 mg/kg/day, po) in corn oil was administered in a volume of 5 ml per kilogram of body weight on gestational days (gd) 6 through 15. Females were weighed and observed during daily treatment and at 1 and 4 hours post-dosing for clinical signs of toxicity. At sacrifice on gestational days 20, a total of 22-27 confirmed-pregnant females per treatment group were evaluated. The gravid uterus for each dam was weighed and the number of implantation sites, and live, dead or resorbed fetuses were recorded. ... During CS treatment, dams exhibited clinical signs including rough or erect coat, lethargy, postural abnormalities, hind limb paralysis and weight loss; clinical signs were most frequent and severe in the 400 and 600 mg/kg/day groups. The maternal mortality rate was 4% (1/25) for the 400 mg/kg/day group and 0% for all other dose groups. On gestational days 11, 15 and 20 maternal body weight was decreased across treatment groups in a dose-related manner with 400 mg/kg/day CS dams exhibiting body weights significantly below vehicle controls on gestational days 15 and 20, and 600 mg/kg/day CS dams exhibiting body weights significantly below controls on gestational days 11, 15 and 20. ... Maternal weight gain during the treatment period was significantly below controls for dams treated with 200, 400 or 600 mg/kg/day CS. Absolute weight gain was significantly below controls for the 400 and 600 mg/kg/day CS groups. Gestational weight gain was below controls for all CS-treated groups. Relative maternal liver weight was increased in a dose-related manner with statistically significant increases above vehicle control observed in the 400 and 600 mg/kg/day CS groups; absolute maternal liver weight did not differ among treatment groups. The percentage per litter of resorbed, dead, nonlive (i.e., dead plus resorbed) or affected (i.e., nonlive plus malformed) fetuses did not differ among dose groups. Among those litters containing live fetuses, there were no differences among dose groups in the number of live fetuses per live litter or in the proportion of males per live litter. Average fetal body weight per live litter was reduced in a dose-related manner, with CS 200, 400 and 600 mg/kg/day litters significantly below controls; males and females were equally affected on this measure. The percentage of fetuses malformed per litter, but not the proportion of litters with one or more malformed fetuses, differed significantly among treatment groups, but no clear dose-effect relationship was observed. In conclusion, CS (0, 100, 200, 400 or 600 mg/kg/day, po) administered on gd 6 through 15, produced dose-related maternal and fetal toxicity, but failed to increase the incidence of malformations in CD rats relative to vehicle control subjects.
[Department of Health & Human Services/National Institute of Environmental Health Sciences, National Toxicology Program; Teratologic Evaluation of Carbon Disulfide (CAS No. 75-15-0) Administered to CD Rats on Gestational Days 6 through 15, NTP Study No. TER84053 (February 24, 1984) available at http://ntp-server.niehs.nih.gov/htdocs/pub-TT0.html as of August 15, 2002]**QC REVIEWED**

Absorption, Distribution & Excretion :

CARBON DISULFIDE CAN REACH FETUSES THROUGH PLACENTA OR BABIES BY WAY OF MOTHER MILK WHEN PREGNANT OR BREAST-FEEDING DURING OR FOLLOWING EXPOSURE.
[CAI SX, BAO YS; IND HEALTH 19 (1): 15 (1981)]**PEER REVIEWED**

Absorption, Distribution & Excretion :

Carbon disulfide blood concentrations reached maximum levels after 2 hours of exposure to about 30 ppm of vapor in air and ranged from 0.15-0.28 mg/l.
[Baselt RC; Biological Monitoring Methods for Industrial Chemicals p.64-7 (1980)]**PEER REVIEWED**

Absorption, Distribution & Excretion :

People not previously exposed absorb about 80% of inhaled vapor during the first 15 minutes, but proportion falls to about 40% after 45 minutes and remains at that level for some time. If workers are previously exposed, about 55% of the inhaled vapor is absorbed during the first 15 minutes. Excretion through the lungs and the urine is small, with about 92% of carbon disulfide retained in the tissues and metabolized.
[Zenz, C., O.B. Dickerson, E.P. Horvath. Occupational Medicine. 3rd ed. St. Louis, MO., 1994722]**PEER REVIEWED**

Metabolism/Metabolites :

A SMALL AMT OF CARBON DISULFIDE IS APPARENTLY CONVERTED TO HYDROGEN SULFIDE, WHICH IS RAPIDLY OXIDIZED TO SULFATE AND EXCRETED IN THE URINE.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-90]**PEER REVIEWED**

Metabolism/Metabolites :

CARBON DISULFIDE REACTS WITH A VARIETY OF NUCLEOPHILIC FUNCTIONAL GROUPS ... AMINO, TO FORM DITHIOCARBAMIC ACIDS ... MERCAPTO, TO FORM TRITHIOCARBAMIC ACIDS ... HYDROXYL, TO FORM XANTHOGENIC ACIDS ... CMPD WITH TWO SUCH GROUPS TO FORM HETEROCYCLES.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-90]**PEER REVIEWED**

Metabolism/Metabolites :

CARBON DISULFIDE ADMIN IP TO RATS WAS OXIDIZED TO (14)CO2 TO AN EXTENT PROPORTIONAL TO THE AMOUNT OF CYTOCHROME P450 PRESENT IN THE LIVER AT THE TIME OF ADMIN.
[The Chemical Society. Foreign Compound Metabolism in Mammals Volume 3. London: The Chemical Society, 1975.429]**PEER REVIEWED**

Metabolism/Metabolites :

Between 10 and 30% of absorbed carbon disulfide is exhaled, less than 1% is excreted in the urine, and the remaining 70-90% undergoes biotransformation before excretion in the urine in the form of sulfur-containing metabolites, only some of which have been identified (eg, thiourea and mercaptothiazolinone).
[WHO; Environ Health Criteria: Carbon Disulfide p.2 (1979)]**PEER REVIEWED**

Biological Half-Life :

Half-life for disappearance of /carbon disulfide/ from blood is estimated at less than 1 hour.
[Baselt RC; Biological Monitoring Methods for Industrial Chemicals p.64 (1980)]**PEER REVIEWED**

Mechanism of Action :

MICROSOMAL METAB OF CARBON-CENTERED THIONON-SULFUR CONTAINING CMPD IS DISCUSSED USING CARBON DISULFIDE, THIOACETAMINE, METHIMAZOLE & ALPHA-NAPHTHYLTHIOUREA AS EXAMPLES. IT IS SUGGESTED THAT COVALENT BINDING OF ATOMIC SULFUR RELEASED IN CYTOCHROME P450 MONOOXYGENASE-CATALYZED METABOLISM OF THIONON-SULFUR COMPOUNDS IS RESPONSIBLE FOR MONOOXYGENASE INHIBITION.
[NEAL RA; REV BIOCHEM TOXICOL 2: 131 (1980)]**PEER REVIEWED**

Interactions :

... SIGNS OF CARBON DISULFIDE POISONING IN ANIMALS ARE INTENSIFIED BY RESERPINE, IPRONIAZID, AND AMPHETAMINE. ...
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-91]**PEER REVIEWED**

Interactions :

... TRYPTOPHAN-ENRICHED DIET INCREASES TOXICITY OF CARBON DISULFIDE. ...
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.III-91]**PEER REVIEWED**

Interactions :

IT IS NOW RECOGNIZED THAT THE NEURAL RESPONSES TO CARBON DISULFIDE ARE GREATLY INFLUENCED BY THE MINERAL CONTENT OF THE DIET, AT LEAST IN ANIMALS. A HIGHLY MINERALIZED DIET OFFERING SUBSTANTIAL PROTECTION FROM NEUROLOGIC EFFECTS.
[American Conference of Governmental Industrial Hygienists, Inc. Documentation of the Threshold Limit Values and Biological Exposure Indices. 6th ed. Volumes I, II, III. Cincinnati, OH: ACGIH, 1991.224]**PEER REVIEWED**

Interactions :

INCLUSION OF EQUIMOLAR MIXT OF CARBON DISULFIDE IN ORAL DOSE OF APPROX 4 LD50'S OF CARBON TETRACHLORIDE (5 MMOL/KG) TO PHENOBARBITONE-PRETREATED RATS REDUCED THE AMT OF LIVER INJURY DUE TO CARBON TETRACHLORIDE & PREVENTED DEATHS.
[SEAWRIGHT AA ET AL; BIOCHEM PHARMACOL 29 (7): 1007 (1980)]**PEER REVIEWED**

Interactions :

In cholesterol fed rabbits carbon disulfide greatly accelerated formation of atheroma.
[Amdur, M.O., J. Doull, C.D. Klaasen (eds). Casarett and Doull's Toxicology. 4th ed. New York, NY: Pergamon Press, 1991.450]**PEER REVIEWED**

Interactions :

Pregnant rats were exposed to 0, 100, 200, 400 or 800 ppm of carbon disulfide (CS2), 100 ppm hydrogen sulfide (H2S) alone or in combination with 400 or 800 ppm CS2, 6 hr/day during days 6-20 gestation. Maternal reproduction and fetal parameters were evaluated on gestational day 21. Treatment with 100 or 200 ppm CS2 or with 100 ppm H2S caused no maternal toxicity or adverse effects on the developing embryo or fetus. Exposure to 400 or 800 ppm CS2 resulted in a low incidence of club foot and in a significant reduction of maternal weight gain. Significant incr in unossified sternebrae occurred at 800 ppm CS2 and reduction of fetal body weight at 400 and 800 ppm CS2. The latter effect was enhanced by combination with 100 ppm H2S. ... At levels of exposure associated with maternal toxicity, CS2 leads to an incr in incidence of club foot and to fetal toxicity which is enhanced by simultaneous exposure to H2S.
[Saillenfait AM, et al; Toxicol Lett 48 (1): 57-66 (1989)]**PEER REVIEWED**

Environmental Abiotic Degradation :

Carbon disulfide hydrolyzes to carbon dioxide and hydrogen disulfide in alkaline solutions(1). The half-life for hydrolysis at pH 9 extrapolated from measurements at higher pH is 1.1 yr (1). It is stable in oxygenated seawater for >10 days(2).
[(1) Peyton TO et al; Carbon Disulfide, Carbonyl Sulfide Literature Review And Environmental Assessment USEPA-600/9-78-009 p. 163 (1976) (2) Lovelock JE; Nature 248: 625-6 (1974)]**PEER REVIEWED**

Environmental Bioconcentration :

BCFs of <6.1 and <60 were measured in carp for carbon disulfide at concentrations of 50 and 5 ug/l, respectively(1). According to a classification scheme(2), these BCFs suggest bioconcentration in aquatic organisms is low to moderate(SRC).
[(1) Chemicals Inspection and Testing Institute; Biodegradation and Bioaccumulation Data of Existing Chemicals Based on the CSCL Japan. Japan Chemical Industry Ecology-Toxicology and Information Center. ISBN #4-89074-101-1 p. 1-1 (1992) (2) Franke C et al; Chemosphere 29: 1501-14 (1994)]**PEER REVIEWED**

Environmental Water Concentrations :

GROUNDWATER: Groundwater samples collected from the Lipari Landfill, NJ in 1984, contained carbon disulfide at <50 ppb(1).
[(1) USEPA; Superfund Record of Decision (EPA Region 7): Lipari Landfill, Mantau Township, Gloucester, NJ, July 11, 1988. Third Remedial Action, Report 1988, EPA/ROD/RO2-88/074; PB89-219224 (1988)]**PEER REVIEWED**

Food Survey Values :

Carbon disulfide was detected in the following fruits and vegetables sampled from a major wholesale market in Sydney, Australia between 1993 and 1995, ug/g (n,% positive): apples, 0.06 to 1.74 (40,82.5%); avocado, <0.02 to 0.12 (16,6.3%); banana, <0.02 (13,0%); beans, 0.07 to 0.68 (25,44.0%); brocol, 0.08 to 1.09 (21,100%); cabbage, 0.04 to 1.37(25,80.0%); bok choy, <0.02 to 0.12 (7,14.3%); capsicum, 0.04 to 0.59 (24,37.5%); carrots, 0.07 to 1.17 (56,21.4%); cauliflower, 0.07 to 1.11 (22,45.5%); celery, 0.06 to 1.70 (22,27.3%); cherries, 0.02 to 0.91 (21,38.1%); chinese cabbage, 0.25 to 2.19 (6,50.0%); citrus, 0.08 to 0.33 (45,6.7%); cucumber, 0.03 to 3.05 (14,71.4%); grapes, 0.08 to 4.11 (45,80.0%); lettuce, 0.07 to 0.77 (39,38.5%); mango, 0.08 to 0.40 (16,37.5%); mushroom, <0.02 to 0.12 (17,5.9%); nectarines, <0.02 to 8.9 (38,47.4%); onion, 0.04 to 4.20 (46,28.3%); peach, 0.07 to 3.31 (36,63.0%); pears, 0.07 to 1.35 (27,63.0%); potato, 0.01 to 0.93 (51,37.3%); rockmelon, 0.02 to 0.1 (24,16.7%); silver beet, 0.03 to 4.68 (20,55.0%); strawberries, 0.004 to 2.50 (24,29.2%); tomato, 0.51 to 1.19 (60,38.3%); zucchini, 0.04 to 0.21 (22,27.3%)(1).
[(1) Ahmad N et al; J AOAC Intl 79: 1417-1422 (1996)]**PEER REVIEWED**

Other Environmental Concentrations :

Carbon disulfide was identified as a volatile compound released from textile floor coverings(1). It was identified in headspace emissions from new carpeting (75% olefin, 25% nylon, polypropylene backing) at approx. 100 ug/cu m(2).
[(1) Sollinger S et al; Atmos Environ 28: 2369-78 (1994) (2) U.S. Consumer Products Safety Commission; Status Report on Chemical Emissions from New Carpet (1993)]**PEER REVIEWED**

Atmospheric Standards :

This action promulgates standards of performance for equipment leaks of Volatile Organic Compounds (VOC) in the Synthetic Organic Chemical Manufacturing Industry (SOCMI). The intended effect of these standards is to require all newly constructed, modified, and reconstructed SOCMI process units to use the best demonstrated system of continuous emission reduction for equipment leaks of VOC, considering costs, non air quality health and environmental impact and energy requirements. Carbon disulfide is produced, as an intermediate or a final product, by process units covered under this subpart.
[40 CFR 60.489 (7/1/97)]**PEER REVIEWED**

Atmospheric Standards :

Listed as a hazardous air pollutant (HAP) generally known or suspected to cause serious health problems. The Clean Air Act, as amended in 1990, directs EPA to set standards requiring major sources to sharply reduce routine emissions of toxic pollutants. EPA is required to establish and phase in specific performance based standards for all air emission sources that emit one or more of the listed pollutants. Carbon disulfide is included on this list.
[Clean Air Act as amended in 1990, Sect. 112 (b) (1) Public Law 101-549 Nov. 15, 1990]**QC REVIEWED**

CERCLA Reportable Quantities :

Releases of CERCLA hazardous substances are subject to the release reporting requirement of CERCLA section 103, codified at 40 CFR part 302, in addition to the requirements of 40 CFR part 355. Carbon Disulfide is an extremely hazardous substance (EHS) subject to reporting requirements when stored in amounts in excess of its threshold planning quantity (TPQ) of 10,000 lbs.
[40 CFR 355 (7/1/97)]**QC REVIEWED**

TSCA Requirements :

Pursuant to section 8(d) of TSCA, EPA promulgated a model Health and Safety Data Reporting Rule. The section 8(d) model rule requires manufacturers, importers, and processors of listed chemical substances and mixtures to submit to EPA copies and lists of unpublished health and safety studies. Carbon disulfide is included on this list.
[40 CFR 716.120 (7/1/97)]**PEER REVIEWED**

RCRA Requirements :

P022; As stipulated in 40 CFR 261.33, when carbon disulfide, as a commercial chemical product or manufacturing chemical intermediate or an off-specification commercial chemical product or a manufacturing chemical intermediate, becomes a waste, it must be managed according to federal and/or state hazardous waste regulations. Also defined as a hazardous waste is any container or inner liner used to hold this waste or any residue, contaminated soil, water, or other debris resulting from the cleanup of a spill, into water or on dry land, of this waste. Generators of small quantities of this waste may qualify for partial exclusion from hazardous waste regulations (40 CFR 261.5(e)).
[40 CFR 261.33 (7/1/97)]**PEER REVIEWED**

RCRA Requirements :

F005; When carbon disulfide is a spent solvent, it is classified as a hazardous waste from a nonspecific source (F005), as stated in 40 CFR 261.31, and must be managed according to State and/or Federal hazardous waste regulations.
[40 CFR 261.31 (7/1/97)]**PEER REVIEWED**

Sampling Procedures :

NIOSH Method 1600. Analyte: Carbon disulfide. Matrix: Air. Sampler: Solid sorbent plus drying tube (coconut shell charcoal, 100 mg/50 mg, and sodium sulfate, 270 mg). Flow Rate: 0.01 to 0.2 l/min. Sample Size: 5 liters. Shipment: Dryer attached to charcoal. Sample Stability: 1 week @ 25 deg C; 6 weeks @ 0 deg C.
[U.S. Department of Health and Human Services, Public Health Service. Centers for Disease Control, National Institute for Occupational Safety and Health. NIOSH Manual of Analytical Methods, 3rd ed. Volumes 1 and 2 with 1985 supplement, and revisions. Washington, DC: U.S. Government Printing Office, February 1984.1600-1]**PEER REVIEWED**

Analytic Laboratory Methods :

Carbon disulfide in biological liquids is analyzed using headspace GC with a GC detector. Retention time 0.40 relative to ethanel, whose absolute retention time is 1.9 + or - 0.1 minutes on a 6'x1/4" od column packed with 5% Carbowax K-600 and 3% Halcomid 18 on 60 to 80 mesh Teflon 6HC.
[Sunshine, Irving (ed.) Methodology for Analytical Toxicology. Cleveland: CRC Press, Inc., 1975.VI/407]**PEER REVIEWED**

Analytic Laboratory Methods :

NIOSH Method 1600. Determination of Carbon Disulfide by Gas Chromatography with Flame Photometric Detection (GC/FID). Detection limit= 2 mg/cu m.
[U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health. NIOSH Manual of Analytical Methods. 4th ed. Methods A-Z & Supplements. Washington, DC: U.S. Government Printing Office, Aug 1994.]**PEER REVIEWED**

Analytic Laboratory Methods :

EPA Method 8015. Direct Injection or Purge-and-Trap Gas Chromatography for the determination of nonhalogenated volatile organics in solid waste. Under the prescribed conditions carbon disulfide can be detected using this method. No statistical analysis was determined; specific method performance information will be provided as it becomes available.
[USEPA/Office of Solid Waste (OSW); Test Methods for Evaluating Solid Waste, Physical/Chemical Methods SW846 Methods (1986)]**PEER REVIEWED**

Analytic Laboratory Methods :

EPA Method 8240. Gas Chromatography/Mass Spectrometry for the determination of volatile Organics. This method can be used to quantify most volatile organic compounds including carbon disulfide that have boiling points below 200 deg C and are insoluble or slightly soluble in water. The detection limit is not given. Precision and method accuracy were found to be directly related to the concentration of the analyte and essentially independent of the sample matrix.
[USEPA/Office of Solid Waste (OSW); Test Methods for Evaluating Solid Waste, Physical/Chemical Methods SW846 Methods (1986)]**PEER REVIEWED**

Special Reports :

COPPOCK RW ET AL; VET HUM TOXICOL 23 (5): 331 (1981). A REVIEW ON TOXICOLOGY OF CARBON DISULFIDE.

Special Reports :

NIOSH; Criteria Document: Carbon Disulfide (1977) DHEW Pub NIOSH 77-156

Special Reports :

DHHS/ATSDR; Toxicological Profile for Carbon Disulfide TP-91/09 (1992)

Special Reports :

Santodonato J; Monograph on Human Exposure to Chemicals in the Workplace: Carbon Disulfide 7 (1986).

GLCC RELATED TOXIC SUBSTANCES FOUND IN THE CAMP POND AND CAMP WATER WELL 2003 AND 2004

GREAT LAKES CHEMICAL CORPORATION AND THE PATHFINDERS CAMP